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BCMO1 Polyclonal antibody

BCMO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23308-1-AP

Synonyms

BCMO1, Beta carotene dioxygenase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using BCMO1 Polyclonal antibody (23308-1-AP)

    WB analysis of COLO 320 using 23308-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 23308-1-AP (BCMO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using BCMO1 Polyclonal antibody (23308-1-AP)

    WB analysis of mouse colon using 23308-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 23308-1-AP (BCMO1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using BCMO1 Polyclonal antibody (23308-1-AP)

    IHC staining of human testis using 23308-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23308-1-AP (BCMO1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inCOLO 320 cells, mouse colon tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23308-1-AP is BCMO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCMO1 fusion protein Ag19732
Full Name beta-carotene 15,15'-monooxygenase 1
Calculated Molecular Weight 547 aa, 63 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC126210
Gene Symbol BCMO1
Gene ID (NCBI) 53630
RRIDAB_2879252
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCMO1(Beta,beta-carotene 15,15'-monooxygenase) is also named as BCDO, BCDO1 and belongs to the carotenoid oxygenase family.The human BCMO1 cDNA encodes a 63-kDa protein with homologies to members of a large and diverse family of polyene chain oxidases and carotenoid cleavage enzymes.Although BCMO1 can be detected in several tissues, its expression is most pronounced in intestinal mucosa and liver(PMID:16504037).It catalyzes the central cleavage of β-carotene to all-trans retinal and is the key enzyme in the intestinal metabolism of carotenes to vitamin A.

Protocols

Product Specific Protocols
WB protocol for BCMO1 antibody 23308-1-APDownload protocol
IHC protocol for BCMO1 antibody 23308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols