Tested Applications
| Positive WB detected in | COLO 320 cells, mouse colon tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23308-1-AP targets BCMO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19732 Product name: Recombinant human BCMO1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 199-547 aa of BC126210 Sequence: FKIPATVPEGKKQGKSPWKHTEVFCSIPSRSLLSPSYYHSFGVTENYVIFLEQPFRLDILKMATAYIRSMSWASCLAFHREEKTYIHIIDQRTRQPVQTKFYTDAMVVFHHVNAYEEDGCIVFDVIAYEDNSLYQLFYLANLNQDFKENSRLTSVPTLRRFAVPLHVDKNAEVGTNLIKVASTTATALKEEDGQVYCQPEFLYEGLELPRVNYAHNGKQYRYVFATGVQWSPIPTKIIKYDILTKSSLKWREDDCWPAEPLFVPAPGAKDEDDGVILSAIVSTDPQKLPFLLILDAKSFTELARASVDVDMHMDLHGLFITDMDWDTKKQAASEEQRDRASDCHGAPLT Predict reactive species |
| Full Name | beta-carotene 15,15'-monooxygenase 1 |
| Calculated Molecular Weight | 547 aa, 63 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC126210 |
| Gene Symbol | BCMO1 |
| Gene ID (NCBI) | 53630 |
| RRID | AB_2879252 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HAY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCMO1(Beta,beta-carotene 15,15'-monooxygenase) is also named as BCDO, BCDO1 and belongs to the carotenoid oxygenase family.The human BCMO1 cDNA encodes a 63-kDa protein with homologies to members of a large and diverse family of polyene chain oxidases and carotenoid cleavage enzymes.Although BCMO1 can be detected in several tissues, its expression is most pronounced in intestinal mucosa and liver(PMID:16504037).It catalyzes the central cleavage of β-carotene to all-trans retinal and is the key enzyme in the intestinal metabolism of carotenes to vitamin A.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BCMO1 antibody 23308-1-AP | Download protocol |
| WB protocol for BCMO1 antibody 23308-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BCMO1 Polyclonal antibody (Cat# 23308-1-AP) has 1 total citation. It appears in Experimental Eye Research (Impact Factor 3.1), published in 2024. The cited study used this antibody for WB and IF applications in human samples, focusing on imaging macular carotenoids and their related proteins in the human retina using confocal resonance Raman and fluorescence microscopy. The research field is ophthalmology and retinal biology, specifically carotenoid metabolism and retinal imaging.
| Species | Application | Title |
|---|---|---|
Exp Eye Res Imaging Macular Carotenoids and Their Related Proteins in the Human Retina with Confocal Resonance Raman and Fluorescence Microscopy |
