BCMO1 Polyclonal antibody

BCMO1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23308-1-AP

Synonyms

BCMO1, Beta carotene dioxygenase 1



Tested Applications

Positive WB detected inCOLO 320 cells, mouse colon tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23308-1-AP is BCMO1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCMO1 fusion protein Ag19732
Full Name beta-carotene 15,15'-monooxygenase 1
Calculated molecular weight 547 aa, 63 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC126210
Gene symbol BCMO1
Gene ID (NCBI) 53630
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCMO1(Beta,beta-carotene 15,15'-monooxygenase) is also named as BCDO, BCDO1 and belongs to the carotenoid oxygenase family.The human BCMO1 cDNA encodes a 63-kDa protein with homologies to members of a large and diverse family of polyene chain oxidases and carotenoid cleavage enzymes.Although BCMO1 can be detected in several tissues, its expression is most pronounced in intestinal mucosa and liver(PMID:16504037).It catalyzes the central cleavage of β-carotene to all-trans retinal and is the key enzyme in the intestinal metabolism of carotenes to vitamin A.

Protocols

Product Specific Protocols
WB protocol for BCMO1 antibody 23308-1-APDownload protocol
IHC protocol for BCMO1 antibody 23308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols