BCO2 Polyclonal antibody

BCO2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14324-1-AP

Synonyms

B DIOX II, BCO2, Beta carotene dioxygenase 2, beta carotene oxygenase 2



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, rat liver tissue
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14324-1-AP targets BCO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCO2 fusion protein Ag5590
Full Name beta-carotene oxygenase 2
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC041656
Gene Symbol BCO2
Gene ID (NCBI) 83875
RRIDAB_2227901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized (PMID:22147584). This antibody detects the N-terminal of BCO2.

Protocols

Product Specific Protocols
WB protocol for BCO2 antibody 14324-1-APDownload protocol
IHC protocol for BCO2 antibody 14324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB,IHC

Proc Natl Acad Sci U S A

Inactivity of human β,β-carotene-9',10'-dioxygenase (BCO2) underlies retinal accumulation of the human macular carotenoid pigment.

Authors - Binxing Li
mouseWB

J Nutr Biochem

Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.

Authors - Xin Guo
mouseWB

Mol Nutr Food Res

Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice.

Authors - Yu Huifeng H
mouseWB

Mol Nutr Food Res

Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: The role of AMP-activated protein kinase α2 subunit.

Authors - Dingbo Lin
mouse

Molecules

Carotenoid Lutein Selectively Inhibits Breast Cancer Cell Growth and Potentiates the Effect of Chemotherapeutic Agents through ROS-Mediated Mechanisms.

Authors - Xiaoming Gong