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  • Featured Product
  • KD/KO Validated

BCR Polyclonal antibody

BCR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22585-1-AP

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, PHL

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of Y79 using 22585-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of HEK-293 using 22585-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of MCF-7 using 22585-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COS-7 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of COS-7 using 22585-1-AP

    COS-7 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of HeLa using 22585-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BCR Polyclonal antibody (22585-1-AP)

    WB analysis of K-562 using 22585-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22585-1-AP (BCR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using BCR Polyclonal antibody (22585-1-AP)

    IP experiment of HEK-293 using 22585-1-AP

    IP result of anti-BCR (IP:22585-1-AP, 4ug; Detection:22585-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

  • Immunohistochemistry (IHC) staining of human skin tissue using BCR Polyclonal antibody (22585-1-AP)

    IHC staining of human skin using 22585-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human skin tissue using BCR Polyclonal antibody (22585-1-AP)

    IHC staining of human skin using 22585-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin slide using 22585-1-AP (BCR Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using BCR Polyclonal antibody (22585-1-AP)

    IF Staining of HeLa using 22585-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22585-1-AP (BCR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BCR Polyclonal antibody (22585-1-AP)

    IF Staining of HepG2 using 22585-1-AP

    Immunofluorescent analysis of HepG2 cells, using BCR antibody 22585-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using BCR Polyclonal antibody (22585-1-AP)

    FC experiment of HepG2 using 22585-1-AP

    1X10^6 HepG2 cells were stained with .2ug BCR antibody (22585-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inY79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22585-1-AP is BCR Fusion Protein expressed in E. coli.

Tested Reactivity human, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCR fusion protein Ag18281
Full Name breakpoint cluster region
Calculated Molecular Weight 143 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC066122
Gene Symbol BCR
Gene ID (NCBI) 613
RRIDAB_2879127
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for BCR antibody 22585-1-APDownload protocol
IHC protocol for BCR antibody 22585-1-APDownload protocol
IF protocol for BCR antibody 22585-1-APDownload protocol
IP protocol for BCR antibody 22585-1-APDownload protocol
FC protocol for BCR antibody 22585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Neurogastroenterol Motil

Loss function of Bcr mutation causes gastrointestinal dysmotility and brain developmental defects.

Authors - Yongtao Xiao
  • KO Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Costanza (Verified Customer) (09-27-2022)

Good Immunofluorescence from paraffin sections

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100