BCR Antibody

BCR Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22585-1-AP

Synonyms

ALL, BCR, breakpoint cluster region, CML, D22S11, D22S662, PHL



Tested Applications

Positive WB detected inY79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 22585-1-AP immunogen is Fusion Protein BCR protein expressed in E. coli.

Tested Reactivity human, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCR fusion protein Ag18281
Full Name breakpoint cluster region
Calculated molecular weight 143 kDa
Observed molecular weight 150-160 kDa
GenBank accession number BC066122
Gene symbol BCR
Gene ID (NCBI) 613
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for BCR antibody 22585-1-APDownload protocol
IHC protocol for BCR antibody 22585-1-APDownload protocol
IF protocol for BCR antibody 22585-1-APDownload protocol
IP protocol for BCR antibody 22585-1-APDownload protocol
FC protocol for BCR antibody 22585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols