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- KD/KO Validated
BST2 Polyclonal antibody
BST2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, FC
Conjugate
Unconjugated
Cat no : 13560-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human heart tissue, L02 cells |
| Positive IHC detected in | human liver tissue, mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| FC | See 1 publications below |
Product Information
13560-1-AP targets BST2 in WB, IHC, IF, FC applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | BST2 fusion protein Ag4430 |
| Full Name | bone marrow stromal cell antigen 2 |
| Calculated molecular weight | 180 aa, 20 kDa |
| Observed molecular weight | 30-36 kDa |
| GenBank accession number | BC033873 |
| Gene symbol | BST2 |
| Gene ID (NCBI) | 684 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BST2 antibody 13560-1-AP | Download protocol |
| IHC protocol for BST2 antibody 13560-1-AP | Download protocol |
| IF protocol for BST2 antibody 13560-1-AP | Download protocol |
| FC protocol for BST2 antibody 13560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biotechnol Lett BST2 promotes cell proliferation, migration and induces NF-κB activation in gastric cancer.
| ||
J Biochem MicroRNA-760 inhibits cell viability and migration through down-regulating BST2 in gastric cancer. | ||
Antiviral Res BST2/Tetherin inhibits hepatitis C virus production in human hepatoma cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Christine (Verified Customer) (10-08-2021) | Detects endogenous BST2 in HeLa cells (BST2 positive), also when overexpressed as FLAG tagged version in HeLa cells. Does no detect anything in HEK293T cells (BST2 negative) but detects many bands when FLAG BST2 or untagged BST2 is overexpressed in these cells.
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