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BST2 Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13560-1-AP

Featured Product KD/KO validated

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Con: 75 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

BST2 Antibody WB human heart tissue 13560-1-AP

human heart tissue were subjected to SDS PAGE followed by western blot with 13560-1-AP(BST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

BST2 Antibody IHC human liver tissue 13560-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 13560-1-AP(BST2 antibody) at dilution of 1:50 (under 10x lens)

BST2 Antibody IHC human liver tissue 13560-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 13560-1-AP(BST2 antibody) at dilution of 1:50 (under 40x lens)

BST2 Antibody IF HepG2 cells 13560-1-AP

Immunofluorescent analysis of HepG2 cells using 13560-1-AP(BST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

BST2 Antibody FC HepG2 cells 13560-1-AP

1X10^6 HepG2 cells were stained with 0.2ug BST2 antibody (13560-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human
Positive WB detected in:
human heart tissue, human liver tissue, L02 cells
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
BST2 fusion protein Ag4430
Full name:
bone marrow stromal cell antigen 2
Calculated molecular weight:
180aa,20 kDa
Observed molecular weight:
30-36 kDa
GenBank accession number:
BC033873
Gene ID (NCBI):
684
Gene symbol
BST2
Synonyms
Bone marrow stromal antigen 2, BST 2, BST2, CD317, HM1.24 antigen, Tetherin

Background

BST2, also named as CD317 and Tetherin, belongs to the tetherin family. It may be involved in the sorting of secreted proteins and it is involved in pre-B-cell growth. BST2 is an antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. BST2 may play a role in B-cell activation in rheumatoid arthritis. It is recently identified interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. BST2 is a plasma membrane proteins, tetherin inhibits virion particle release from infected cells. BST2 is effective against retroviruses and flavoviruses whilst IFITMs disrupt influenza and flavivirus infection. Observed MW of BST2 is 30-36 kDa (PMID: 19196977; 21237475).


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