C1qC Polyclonal antibody
C1qC Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16889-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, mouse serum |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
16889-1-AP targets C1qC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C1qC fusion protein Ag10400 |
| Full Name | complement component 1, q subcomponent, C chain |
| Calculated Molecular Weight | 245 aa, 26 kDa |
| Observed Molecular Weight | 24-26 kDa |
| GenBank Accession Number | BC009016 |
| Gene Symbol | C1QC |
| Gene ID (NCBI) | 714 |
| RRID | AB_2878328 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C1qC antibody 16889-1-AP | Download protocol |
| IHC protocol for C1qC antibody 16889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
World J Surg Oncol Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma | ||
Front Genet C1QC is a prognostic biomarker with immune-related value in kidney renal clear cell carcinoma
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