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SLC52A3 Polyclonal antibody

SLC52A3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25626-1-AP

Synonyms

C20orf54, hRFT2, RFT2, RFVT3, Riboflavin transporter 2, SLC52A3, Solute carrier family 52, riboflavin transporter, member 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SLC52A3 Polyclonal antibody (25626-1-AP)

    WB analysis of mouse brain using 25626-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25626-1-AP (SLC52A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SLC52A3 Polyclonal antibody (25626-1-AP)

    IHC staining of human testis using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25626-1-AP (SLC52A3 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SLC52A3 Polyclonal antibody (25626-1-AP)

    IHC staining of human testis using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25626-1-AP (SLC52A3 antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC52A3 Polyclonal antibody (25626-1-AP)

    IHC staining of mouse testis using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SLC52A3 Polyclonal antibody (25626-1-AP)

    IHC staining of mouse testis using 25626-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25626-1-AP (SLC52A3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25626-1-AP is SLC52A3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC52A3 fusion protein Ag22315
Full Name Solute carrier family 52, riboflavin transporter, member 3
Calculated Molecular Weight 469 aa, 51 kDa
GenBank Accession NumberBC009750
Gene Symbol C20orf54
Gene ID (NCBI) 113278
RRIDAB_2880167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC52A3 antibody 25626-1-APDownload protocol
IHC protocol for SLC52A3 antibody 25626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols