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C9orf86 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 20848-1-AP

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Con: 49 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

C9orf86 Antibody WB MDA-MB-453s cells 20848-1-AP

MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP( C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

C9orf86 Antibody IHC human breast cancer tissue 20848-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP( C9orf86 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

C9orf86 Antibody IHC human breast cancer tissue 20848-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP( C9orf86 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

C9orf86 Antibody IF HeLa cells 20848-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HeLa cells using 20848-1-AP(C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human
Positive WB detected in:
MDA-MB-453s cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
C9orf86 fusion protein Ag14350
Full name:
chromosome 9 open reading frame 86
Calculated molecular weight:
729aa,80 kDa
Observed molecular weight:
125 kDa
GenBank accession number:
BC002945
Gene ID (NCBI):
55684
Gene symbol
C9orf86
Synonyms
C9orf86, Parf, Partner of ARF, pp8875, RBEL1

Background

C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]


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