Tested Applications
| Positive WB detected in | MDA-MB-453s cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
20848-1-AP targets C9orf86 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | C9orf86 fusion protein Ag14350 Predict reactive species |
| Full Name | chromosome 9 open reading frame 86 |
| Calculated Molecular Weight | 729 aa, 80 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | BC002945 |
| Gene Symbol | C9orf86 |
| Gene ID (NCBI) | 55684 |
| RRID | AB_2878750 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3YEC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for C9orf86 antibody 20848-1-AP | Download protocol |
| IHC protocol for C9orf86 antibody 20848-1-AP | Download protocol |
| IF protocol for C9orf86 antibody 20848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
