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C9orf86 Polyclonal antibody

C9orf86 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20848-1-AP

Synonyms

C9orf86, Parf, Partner of ARF, pp8875, RBEL1

Validation Data Gallery

  • WB analysis of MDA-MB-453s using 20848-1-AP

    WB analysis of MDA-MB-453s using 20848-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 20848-1-AP (C9orf86 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 20848-1-AP

    IHC staining of human breast cancer using 20848-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20848-1-AP (C9orf86 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 20848-1-AP

    IF Staining of HeLa using 20848-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20848-1-AP (C9orf86 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMDA-MB-453s cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20848-1-AP is C9orf86 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C9orf86 fusion protein Ag14350
Full Name chromosome 9 open reading frame 86
Calculated molecular weight 729 aa, 80 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC002945
Gene symbol C9orf86
Gene ID (NCBI) 55684
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C9orf86, also known as RBEL1 (Rab-like protein 1), is a novel subfamily of GTPases within the Ras superfamily. It has two splice variants, RBEL1Aand RBEL1B. Unlike known Rabs that aremostly cytosolic, RBEL1B predominantly resides in the nucleus, whereas RBEL1A is localized primarily to the cytosol. C9orf86 is overexpressed in the majority of primary breast tumors, and knockdown of C9orf86 in MCF-7 breast cancer cells resulted in cell growth suppression associated with apoptosis [PMID: 23977139]. Western blot analysis revealed that the endogenous RBEL1 migrated at 110-125 kDa, 80 kDa, maybe due to a number of potential O-linked glycosylation sites.[PMID: 17962191]

Protocols

Product Specific Protocols
WB protocol for C9orf86 antibody 20848-1-APDownload protocol
IHC protocol for C9orf86 antibody 20848-1-APDownload protocol
IF protocol for C9orf86 antibody 20848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols