CA1 Polyclonal antibody

CA1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13198-2-AP

Synonyms

CA I, CA1, CAB, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, carbonic anhydrase I



Tested Applications

Positive WB detected inmouse spleen tissue, Jurkat cells, rat spleen tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13198-2-AP targets CA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA1 fusion protein Ag3932
Full Name carbonic anhydrase I
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC027890
Gene Symbol CA1
Gene ID (NCBI) 759
RRIDAB_2275041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

Protocols

Product Specific Protocols
WB protocol for CA1 antibody 13198-2-APDownload protocol
IHC protocol for CA1 antibody 13198-2-APDownload protocol
IP protocol for CA1 antibody 13198-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,ratWB,IF

Acta Neuropathol Commun

Upregulation of carbonic anhydrase 1 beneficial for depressive disorder

Authors - Ke Cheng
  • KO Validated
ratWB

J Ginseng Res

Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway

Authors - Mei-Li Lu
mouseIF

FASEB J

Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.

Authors - Brittany R Jenkins
humanWB

J Inflamm Res

M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs

Authors - Xianqin Song
mouseWB

Am J Physiol Lung Cell Mol Physiol

Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.

Authors - Kathleen Boyne
human,ratWB

Front Pharmacol

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Authors - Meili Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Brittany (Verified Customer) (09-27-2019)

Using the CA-1 primary antibody with mouse colon scrapes yielded very clean bands with western blotting. Immunofluorescence also gave a fairly clean, detectable signal, with CA-1 showing up along the brush border in mouse colon tissue. There were some cells that stained more intracellularly, and unsure if that was non-specific binding of the antibody, or another cell type other than colonocytes that expresses CA-1 intracellularly.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000 (WB) and 1:100 (IF)
  • Cell Tissue Type: Colon tissue (scrapes for western blot)
CA1 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB) and 1:100 (IF) dilution) in Colon tissue (scrapes for western blot) (Cat no:13198-2-AP)