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CA12 Polyclonal antibody

CA12 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 15180-1-AP


CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3

Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15180-1-AP targets CA12 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA12 fusion protein Ag7107
Full Name carbonic anhydrase XII
Calculated molecular weight 39 kDa
Observed molecular weight 44 kDa, 75-80 kDa
GenBank accession numberBC000278
Gene symbol CA12
Gene ID (NCBI) 771
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.


Product Specific Protocols
WB protocol for CA12 antibody 15180-1-APDownload protocol
IHC protocol for CA12 antibody 15180-1-APDownload protocol
FC protocol for CA12 antibody 15180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


mouseWB, IF

J Physiol

Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.

Authors - Jeong Hee Hong

J Enzyme Inhib Med Chem

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.

Authors - Soyoung Hwang
humanWB, IP

Channels (Austin)

Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity.

Authors - Yon Soo Jeong


Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration.

Authors - Soyoung Hwang


PEGylated anticancer-carbon nanotubes complex targeting mitochondria of lung cancer cells.

Authors - Sang-Woo Kim

Cell Physiol Biochem

N-Cadherin Maintains the Healthy Biology of Nucleus Pulposus Cells under High-Magnitude Compression.

Authors - Zhenyu Wang