• Featured Product
  • KD/KO Validated

CA12 Polyclonal antibody

CA12 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15180-1-AP

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3



Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Sample-dependent, check data in validation data gallery

Product Information

15180-1-AP targets CA12 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA12 fusion protein Ag7107
Full Name carbonic anhydrase XII
Calculated molecular weight 39 kDa
Observed molecular weight 44 kDa, 75-80 kDa
GenBank accession numberBC000278
Gene symbol CA12
Gene ID (NCBI) 771
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Protocols

Product Specific Protocols
WB protocol for CA12 antibody 15180-1-APDownload protocol
IHC protocol for CA12 antibody 15180-1-APDownload protocol
FC protocol for CA12 antibody 15180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB, IF

J Physiol

Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.

Authors - Jeong Hee Hong
humanWB,IF

J Enzyme Inhib Med Chem

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.

Authors - Soyoung Hwang
humanWB, IP

Channels (Austin)

Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity.

Authors - Yon Soo Jeong
humanWB,IF

Molecules

Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration.

Authors - Soyoung Hwang
humanWB

Nanotechnology

PEGylated anticancer-carbon nanotubes complex targeting mitochondria of lung cancer cells.

Authors - Sang-Woo Kim
ratWB

Cell Physiol Biochem

N-Cadherin Maintains the Healthy Biology of Nucleus Pulposus Cells under High-Magnitude Compression.

Authors - Zhenyu Wang