|Positive WB detected in||A549 cells, BxPC-3 cells, MCF-7 cells|
|Positive IHC detected in||human pancreas tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Sample-dependent, check data in validation data gallery|
15180-1-AP targets CA12 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CA12 fusion protein Ag7107|
|Full Name||carbonic anhydrase XII|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||44 kDa, 75-80 kDa|
|GenBank accession number||BC000278|
|Gene ID (NCBI)||771|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.
J Enzyme Inhib Med Chem
Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.
Governing effect of regulatory proteins for Cl(-)/HCO3(-) exchanger 2 activity.
Drug Repurposing as an Antitumor Agent: Disulfiram-Mediated Carbonic Anhydrase 12 and Anion Exchanger 2 Modulation to Inhibit Cancer Cell Migration.
PEGylated anticancer-carbon nanotubes complex targeting mitochondria of lung cancer cells.
Cell Physiol Biochem
N-Cadherin Maintains the Healthy Biology of Nucleus Pulposus Cells under High-Magnitude Compression.