Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human placenta tissue, human heart tissue, human kidney tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
16696-1-AP targets CA13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CA13 fusion protein Ag10086 Predict reactive species |
| Full Name | carbonic anhydrase XIII |
| Calculated Molecular Weight | 262 aa, 29 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC052602 |
| Gene Symbol | CA13 |
| Gene ID (NCBI) | 377677 |
| RRID | AB_1850972 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N1Q1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CA13 antibody 16696-1-AP | Download protocol |
| IHC protocol for CA13 antibody 16696-1-AP | Download protocol |
| IF protocol for CA13 antibody 16696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Med Genomics Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4-/- mouse | ||
Anal Chem Spike-In Proteome Enhances Data-Independent Acquisition for Thermal Proteome Profiling |
