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CA13 Polyclonal antibody

CA13 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16696-1-AP

Synonyms

CA XIII, CA13, Carbonate dehydratase XIII, Carbonic anhydrase 13, carbonic anhydrase XIII, CAXIII

Validation Data Gallery

  • WB analysis of NIH/3T3 using 16696-1-AP

    WB analysis of NIH/3T3 using 16696-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16696-1-AP (CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 16696-1-AP

    IHC staining of human placenta using 16696-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human placenta using 16696-1-AP

    IHC staining of human placenta using 16696-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16696-1-AP (CA13 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of NIH/3T3 using 16696-1-AP

    IF Staining of NIH/3T3 using 16696-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16696-1-AP (CA13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inNIH/3T3 cells, mouse skeletal muscle tissue
Positive IHC detected inhuman placenta tissue, human heart tissue, human kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16696-1-AP targets CA13 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA13 fusion protein Ag10086
Full Name carbonic anhydrase XIII
Calculated molecular weight 262 aa, 29 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC052602
Gene symbol CA13
Gene ID (NCBI) 377677
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic proteinmay that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).

Protocols

Product Specific Protocols
WB protocol for CA13 antibody 16696-1-APDownload protocol
IHC protocol for CA13 antibody 16696-1-APDownload protocol
IF protocol for CA13 antibody 16696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols