Name: CA13 Rabbit Polyclonal antibody
Brand: Proteintech

Tested Applications

Applications:

WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee

CA13 Antibody WB NIH/3T3 cells 16696-1-AP

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16696-1-AP(CA13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

CA13 Antibody IHC human placenta tissue 16696-1-AP

Immunohistochemical of paraffin-embedded human placenta using 16696-1-AP(CA13 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human placenta using 16696-1-AP(CA13 antibody) at dilution of 1:100 (under 40x lens)

CA13 Antibody IF NIH/3T3 cells 16696-1-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 16696-1-AP(CA13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:

human, mouse, rat

Positive WB detected in:

NIH/3T3 cells, mouse skeletal muscle tissue

Positive IHC detected in:

human placenta tissue, human heart tissue, human kidney tissue, human spleen tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

NIH/3T3 cells

Recommended dilution:

WB : 1:500-1:3000

IHC : 1:20-1:200

IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

CA13 fusion protein Ag10086

Full name:

carbonic anhydrase XIII

Calculated molecular weight:

262aa,29 kDa

Observed molecular weight:

30 kDa

GenBank accession number:

BC052602

Gene ID (NCBI):

377677

Gene symbol

CA13

Synonyms

CA XIII, CA13, Carbonate dehydratase XIII, Carbonic anhydrase 13, carbonic anhydrase XIII, CAXIII

Background

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic proteinmay that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).