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- KD/KO Validated
MUC1/CA15-3 N-terminal Polyclonal antibody
MUC1/CA15-3 N-terminal Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Cat no : 19976-1-AP
|Positive WB detected in||A431 cells|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue, human meningioma tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
19976-1-AP targets MUC1/CA15-3 N-terminal in WB, IP, IHC, FC, CoIP,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Full Name||mucin 1, cell surface associated|
|Calculated molecular weight||122 kDa|
|Observed molecular weight||122 kDa|
|GenBank accession number||J05582|
|Gene ID (NCBI)||4582|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the N-terminal region of human MUC1, thus it recognizes the 120 kDa extracellular MUC1.
|Product Specific Protocols|
|WB protocol for MUC1/CA15-3 N-terminal antibody 19976-1-AP||Download protocol|
|IHC protocol for MUC1/CA15-3 N-terminal antibody 19976-1-AP||Download protocol|
|IP protocol for MUC1/CA15-3 N-terminal antibody 19976-1-AP||Download protocol|
|FC protocol for MUC1/CA15-3 N-terminal antibody 19976-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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