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MUC1/CA15-3 N-terminal Antibody

MUC1/CA15-3 N-terminal Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19976-1-AP

Synonyms

CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin



Tested Applications

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human meningioma tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

19976-1-AP targets MUC1/CA15-3 N-terminal in WB, IP, IHC, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mucin 1, cell surface associated
Calculated molecular weight 122 kDa
Observed molecular weight 122 kDa
GenBank accession numberJ05582
Gene symbol MUC1
Gene ID (NCBI) 4582
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the N-terminal region of human MUC1, thus it recognizes the 120 kDa extracellular MUC1.

Protocols

Product Specific Protocols
WB protocol for MUC1/CA15-3 N-terminal antibody 19976-1-APDownload protocol
IHC protocol for MUC1/CA15-3 N-terminal antibody 19976-1-APDownload protocol
IP protocol for MUC1/CA15-3 N-terminal antibody 19976-1-APDownload protocol
FC protocol for MUC1/CA15-3 N-terminal antibody 19976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Endocr Pathol

Expression of MUC1 and CD176 (Thomsen-Friedenreich antigen) in Papillary Thyroid Carcinomas.

Authors - Xiang-Xiang Zhan
humanWB

J Exp Clin Cancer Res

CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182.

Authors - Zhi-Hong Zong
humanWB

Reprod Biol Endocrinol

PMID: 29925391

Authors - Cheng Li
humanIHC

Hum Cell

GalNAc-T3 and MUC1, a combined predictor of prognosis and recurrence in solitary pulmonary adenocarcinoma initially diagnosed as malignant solitary pulmonary nodule (≤ 3 cm).

Authors - Qiang Xie
humanWB

Lab Invest

Endogenous and exogenous galectin-3 promote the adhesion of tumor cells with low expression of MUC1 to HUVECs through upregulation of N-cadherin and CD44.

Authors - Zhanqi Cao
humanWB,CoIP

Oncogene

Core 3 mucin-type O-glycan restoration in colorectal cancer cells promotes MUC1/p53/miR-200c-dependent epithelial identity.

Authors - J Ye
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