CA2 Polyclonal antibody

CA2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16961-1-AP

Synonyms

CA II, CA2, CAC, CAII, Car2, Carbonate dehydratase II, Carbonic anhydrase 2, Carbonic anhydrase C, carbonic anhydrase II



Tested Applications

Positive WB detected inHEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

16961-1-AP targets CA2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA2 fusion protein Ag10680
Full Name carbonic anhydrase II
Calculated molecular weight 260 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC011949
Gene symbol CA2
Gene ID (NCBI) 760
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).

Protocols

Product Specific Protocols
WB protocol for CA2 antibody 16961-1-APDownload protocol
IP protocol for CA2 antibody 16961-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Physiol

Essential role of carbonic anhydrase XII in secretory glands fluid and HCO3-Secretion revealed by disease causing human mutation.

Authors - Jeong Hee Hong
humanWB,IHC

Sci Rep

SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.

Authors - Yanzhang Luo
mouseWB

Mol Cell Proteomics

Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.

Authors - Khositseth Sookkasem S

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Isabel (Verified Customer) (07-03-2020)

Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary mouse osteoclasts
CA2 Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:16961-1-AP)