CA4 Polyclonal antibody

CA4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13931-1-AP

Synonyms

CA IV, CA4, CAIV, Car4, Carbonate dehydratase IV, Carbonic anhydrase 4, carbonic anhydrase IV, RP17



Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13931-1-AP targets CA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA4 fusion protein Ag4960
Full Name carbonic anhydrase IV
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC057792
Gene symbol CA4
Gene ID (NCBI) 762
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

Protocols

Product Specific Protocols
WB protocol for CA4 antibody 13931-1-APDownload protocol
IHC protocol for CA4 antibody 13931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols