CA5B Polyclonal antibody

CA5B Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13342-1-AP

Synonyms

CA VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB



Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

13342-1-AP targets CA5B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA5B fusion protein Ag4166
Full Name carbonic anhydrase VB, mitochondrial
Calculated molecular weight 317 aa, 36 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC028142
Gene symbol CA5B
Gene ID (NCBI) 11238
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA5B(Carbonic anhydrase 5B, mitochondrial) is also named as CA-VB and belongs to the alpha-carbonic anhydrase family.CA5B encodes a 317-amino acid protein that has 64% sequence similarity with CA5 (114761), a mitochondrial CA, and contains a 33-amino-acid residue signal sequence. Western blot analysis revealed a 36-kD protein precursor and a 32-kD mature protein(PMID:10409679).

Protocols

Product Specific Protocols
WB protocol for CA5B antibody 13342-1-APDownload protocol
IHC protocol for CA5B antibody 13342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols