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CA7 Polyclonal antibody

CA7 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13670-1-AP

Synonyms

CA VII, CA7, Carbonate dehydratase VII, Carbonic anhydrase 7, carbonic anhydrase VII, CAVII

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CA7 Polyclonal antibody (13670-1-AP)

    WB analysis using 13670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CA7 Polyclonal antibody (13670-1-AP)

    WB analysis using 13670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human liver cancer using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human kidney using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human kidney using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human stomach using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CA7 Polyclonal antibody (13670-1-AP)

    IHC staining of human stomach using 13670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CA7 Polyclonal antibody (13670-1-AP)

    IF Staining of HeLa using 13670-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13670-1-AP targets CA7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA7 fusion protein Ag4649
Full Name carbonic anhydrase VII
Calculated Molecular Weight 264 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC033865
Gene Symbol CA7
Gene ID (NCBI) 766
RRIDAB_2243629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CA7 antibody 13670-1-APDownload protocol
IHC protocol for CA7 antibody 13670-1-APDownload protocol
IF protocol for CA7 antibody 13670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,rat,pigIF

Cell Regen

Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium.

Authors - Haonan Li
View Article