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CA8 Polyclonal antibody

CA8 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12391-1-AP

Synonyms

CA VIII, CA8, CALS, carbonic anhydrase VIII, CARP



Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12391-1-AP targets CA8 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA8 fusion protein Ag3068
Full Name carbonic anhydrase VIII
Calculated molecular weight 290 aa, 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC015531
Gene symbol CA8
Gene ID (NCBI) 767
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The CA8 (CARP) gene encodes carbonic anhydrase VIII, which is part of a family of zinc metalloenzyme. CA8 has a central carbonic anhydrase motif, but it lacks carbonic anhydrase activity due to absence of catalytic zinc coordinating residues(PMID:2121526). CARP is a novel IP3R1-binding protein, and is expressed in Purkinje cells abundantly. CA8 is co-localized with IP3R1 in Purkinje cells and it binds to IP3R1, reducing the affinity of the receptor for its ligand, IP3(PMID:12611586). Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3)(PMID:19461874).

Protocols

Product Specific Protocols
WB protocol for CA8 antibody 12391-1-APDownload protocol
IHC protocol for CA8 antibody 12391-1-APDownload protocol
IP protocol for CA8 antibody 12391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

PLoS One

Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.

Authors - Liddelow Shane A SA
humanWB,IHC

Biomed Pharmacother

CA8 promotes RCC proliferation and migration though its expression level is lower in tumor compared to adjacent normal tissue.

Authors - Huai-Lu Ma
  • KD Validated