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CA9 Polyclonal antibody

CA9 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11071-1-AP

Synonyms

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman stomach tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11071-1-AP targets CA9 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA9 fusion protein Ag1540
Full Name carbonic anhydrase IX
Calculated molecular weight 459 aa, 50 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC014950
Gene symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549(JOC,vol21,no.3,473-482).

Protocols

Product Specific Protocols
WB protocol for CA9 antibody 11071-1-APDownload protocol
IHC protocol for CA9 antibody 11071-1-APDownload protocol
IP protocol for CA9 antibody 11071-1-APDownload protocol
FC protocol for CA9 antibody 11071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Loss of Phd2 cooperates with BRAFV600E to drive melanomagenesis.

Authors - Shujing Liu
humanIHC

Pathol Int

Succinate dehydrogenase B-deficient renal cell carcinoma: A case report with novel germline mutation.

Authors - Hiromichi Iwashita
humanIHC

J Comput Assist Tomogr

Necrosis degree displayed in computed tomography images correlated with hypoxia and angiogenesis in breast cancer.

Authors - Shan Xiuhong X
humanWB

Am J Transl Res

Comprehensive analysis of differentially expressed non-coding RNAs and mRNAs in gastric cancer cells under hypoxic conditions.

Authors - Jia Li
humanWB

Oncol Rep

PMID: 31436301

Authors - Ji-Min Li
mouseWB

PLoS One

The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model.

Authors - Eric C Woolf

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paulo (Verified Customer) (03-04-2019)

Total cell lysate (50 ug of human ccRCC cell lines) was resolved on 10% Bis-Tris gel and transferred to PVDF membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CAIX in blocking buffer (1:250) at 4C overnight. After 1h incubation with appropriate secondary antibody (DAKO anti-Rabbit 1:10000) membranes were imaged with ECL and expected band was detected

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Human kidney cancer cells