|Positive WB detected in||RAW264.7, Raji cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, C6 cells, Jurkat cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22915-1-AP is Caspase 1/p20/p10 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, monky, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 1/p20/p10 fusion protein Ag11143|
|Full Name||caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)|
|Calculated molecular weight||404 aa, 45 kDa|
|Observed molecular weight||45-47 kDa, 30 kDa, 35 kDa|
|GenBank accession number||BC062327|
|Gene ID (NCBI)||834|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20..
|Product Specific Protocols|
|WB protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|IHC protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|IF protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Int J Nanomedicine
Porous Se@SiO2 nanospheres attenuate ischemia/reperfusion (I/R)-induced acute kidney injury (AKI) and inflammation by antioxidative stress.
Am J Transl Res
Muscone improves cardiac function in mice after myocardial infarction by alleviating cardiac macrophage-mediated chronic inflammation through inhibition of NF-κB and NLRP3 inflammasome.
The selective NLRP3 inflammasome inhibitor MCC950 alleviates cholestatic liver injury and fibrosis in mice.
Oxid Med Cell Longev
Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice.
Mitophagy inhibits amyloid-β and tau pathology and reverses cognitive deficits in models of Alzheimer's disease.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joseph (Verified Customer) (02-19-2020)
This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.