Caspase 12 Polyclonal antibody

Caspase 12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55238-1-AP

Synonyms

CASP 12, CASP12, CASP12P1, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12



Tested Applications

Positive WB detected inHEK-293 cells, multi-cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate hyperplasia tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55238-1-AP targets Caspase 12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity hamster, hamsters, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name caspase 12 (gene/pseudogene)
Calculated molecular weight 39 kDa
Observed molecular weight 36-42 kDa, 50 kDa
GenBank accession numberNR_000035
Gene symbol CASP12
Gene ID (NCBI) 120329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

Protocols

Product Specific Protocols
WB protocol for Caspase 12 antibody 55238-1-APDownload protocol
IHC protocol for Caspase 12 antibody 55238-1-APDownload protocol
IF protocol for Caspase 12 antibody 55238-1-APDownload protocol
IP protocol for Caspase 12 antibody 55238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gene

Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.

Authors - Zhaodong Li
ratWB

J Cell Physiol

Sensory plasticity of carotid body is correlated with oxidative stress in paraventricular nucleus during chronic intermittent hypoxia.

Authors - Pei Liu
mouseWB

Oxid Med Cell Longev

Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca2+ Imbalance-Mediated Endoplasmic Reticulum Stress.

Authors - Jiahui Cui
hamstersWB

Int J Mol Sci

PMID: 30845718

Authors - Syed Zahid Ali Shah
ratWB,IHC

Toxicol Lett

Bone marrow mesenchymal stem cells could reduce the toxic effects of hexavalent chromium on the liver by decreasing endoplasmic reticulum stress-mediated apoptosis via SIRT1/HIF-1α signaling pathway in rats.

Authors - Yue Zhao
ratWB

Biomed Pharmacother

HBOA ameliorates CCl4-incuded liver fibrosis through inhibiting TGF-β1/Smads, NF-κB and ERK signaling pathways.

Authors - Xuemei Sun