Validation Data Gallery
|Positive WB detected in||HEK-293 cells, multi-cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human prostate hyperplasia tissue, human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55238-1-AP targets Caspase 12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||hamster, hamsters, human|
|Host / Isotype||Rabbit / IgG|
|Full Name||caspase 12 (gene/pseudogene)|
|Calculated molecular weight||39 kDa|
|Observed molecular weight||36-42 kDa, 50 kDa|
|GenBank accession number||NR_000035|
|Gene ID (NCBI)||120329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.
|Product Specific Protocols|
|WB protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IHC protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IF protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|IP protocol for Caspase 12 antibody 55238-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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