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  • KD/KO Validated

Calcium Sensing Receptor Antibody

Calcium Sensing Receptor Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19125-1-AP

Synonyms

Calcium Sensing Receptor, CAR, CASR, FHH, FIH, GPRC2A, HHC, HHC1, NSHPT, PCAR1



Tested Applications

Positive WB detected inCOLO 320 cells, HL-60 cells, Jurkat cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19125-1-AP targets Calcium Sensing Receptor in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name calcium-sensing receptor
Calculated molecular weight 121 kDa
Observed molecular weight 140 kDa, 60-65 kDa
GenBank accession number NM_000388
Gene symbol CASR
Gene ID (NCBI) 846
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CASR, also named as GPRC2A, PCAR1, belongs to the G-protein coupled receptor 3 family. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. The activity of CASR is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo). The 60-65 kDa band detected by this antibody might represent a degradation product of CASR (PMID: 15718278).

Protocols

Product Specific Protocols
WB protocol for Calcium Sensing Receptor antibody 19125-1-APDownload protocol
IF protocol for Calcium Sensing Receptor antibody 19125-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Biol Pharm Bull

Calhex231 alleviates high glucose-induced myocardial fibrosis via inhibiting Itch-ubiquitin proteasome pathway in Vitro.

Authors - Hui Yuan
humanWB

Am J Physiol Cell Physiol

miR-429 and miR-424-5p inhibit cell proliferation and Ca2+ influx by downregulating CaSR in pulmonary artery smooth muscle cells.

Authors - Chuang Li
  • KD Validated
ratIHC

Hum Exp Toxicol

Role of autophagy in cadmium-induced testicular injury.

Authors - Y-J Wang
humanWB,IHC

Acta Pharmacol Sin

Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.

Authors - Bo Yin
human,ratWB,IF

Front Pharmacol

Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.

Authors - Meili Lu