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CBLN1 Polyclonal antibody

CBLN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18207-1-AP

Synonyms

CBLN1, CER [des Ser1] cerebellin, Cerebellin 1, cerebellin 1 precursor, Precerebellin

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using CBLN1 Polyclonal antibody (18207-1-AP)

    WB analysis of SH-SY5Y using 18207-1-AP

    WB result of CBLN1 antibody (18207-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBLN1 transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of various lysates using CBLN1 Polyclonal antibody (18207-1-AP)

    WB analysis using 18207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18207-1-AP (CBLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSH-SY5Y cells, U-87 MG cells, Neuro-2a cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18207-1-AP targets CBLN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CBLN1 fusion protein Ag12835
Full Name cerebellin 1 precursor
Calculated Molecular Weight 193 aa, 21 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC093692
Gene Symbol CBLN1
Gene ID (NCBI) 869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBLN1 (Cerebellin-1) is also named as Precerebellin. CBLN1 is a newly identified synaptic organizer belonging to the C1q family. Cbln1 was recently identified as the missing ligand for the orphan glutamate receptor δ2 (GluD2) (PMID: 21342763). In the cerebellum, Cbln1 is produced and secreted from granule cells and works as a strong synapse organizer between Purkinje cells and parallel fibers, the axons of the granule cells (PMID: 24607546). The glycosylation modification of CBLN1 resulted in a larger molecular weight.

Protocols

Product Specific Protocols
WB protocol for CBLN1 antibody 18207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols