CCL28 Polyclonal antibody

CCL28 Polyclonal Antibody for FC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 18214-1-AP

Synonyms

C C motif chemokine 28, CCK1, CCL28, MEC, Protein CCK1, SCYA28, Small inducible cytokine A28



Tested Applications

Positive WB detected inhuman spleen tissue, Jurkat cells, mouse spleen tissue, PC-3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18214-1-AP targets CCL28 in WB, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCL28 fusion protein Ag12920
Full Name chemokine (C-C motif) ligand 28
Calculated molecular weight 127 aa, 14 kDa
Observed molecular weight 8 kDa-9 kDa
GenBank accession numberBC069532
Gene symbol CCL28
Gene ID (NCBI) 56477
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCL28, also named as SCYA28, CCK1 and MEC, belongs to the intercrine beta (chemokine CC) family. It has chemotactic activity for resting CD4, CD8 T-cells and eosinophils. CCL28 binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner. CCL28 is expressed in a variety of human and mouse tissues, and it appears to be predominantly produced by epithelial cells. It is produced by epithelial cells of these tissues suggesting that this chemokine can play an important role by linking homing mechanisms between the gut, nasal mucosa and mammary gland (MG). In WB test, CCL28 is 14kd and 8-9kd(a putatively to a degradation fragment).

Protocols

Product Specific Protocols
WB protocol for CCL28 antibody 18214-1-APDownload protocol
IHC protocol for CCL28 antibody 18214-1-APDownload protocol
FC protocol for CCL28 antibody 18214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Cancer Res

CCL27/CCL28-CCR10 CHEMOKINE SIGNALING MEDIATES MIGRATION OF LYMPHATIC ENDOTHELIAL CELLS.

Authors - Tara Karnezis
humanWB

Oncol Rep

CCL28 promotes breast cancer growth and metastasis through MAPK-mediated cellular anti-apoptosis and pro-metastasis.

Authors - Xiao Li Yang