CD163 Polyclonal antibody

CD163 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 16646-1-AP

Synonyms

CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163



Tested Applications

Positive WB detected inpig spleen tissue, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human lung tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16646-1-AP is CD163 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD163 fusion protein Ag10029
Full Name CD163 molecule
Calculated molecular weight 1156 aa, 125 kDa
Observed molecular weight 130-145 kDa
GenBank accession numberBC051281
Gene symbol CD163
Gene ID (NCBI) 9332
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.

Protocols

Product Specific Protocols
WB protocol for CD163 antibody 16646-1-APDownload protocol
IHC protocol for CD163 antibody 16646-1-APDownload protocol
FC protocol for CD163 antibody 16646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF,IHC

Int J Cancer

Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma.

Authors - Qun Chen
humanIHC

J Ethnopharmacol

The effect of Jianpi Yangzheng Xiaozheng Decoction and its components on gastric cancer.

Authors - Jian Wu
humanWB,FC

Am J Physiol Cell Physiol

OSCC-derived exosomes promote M2-type macrophage polarization mediated by exosome-enclosed miR-29a-3p.

Authors - Jinghua Cai
humanIHC,IF

EBioMedicine

DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma.

Authors - Xiangqi Meng
mouseIF

Biochem Pharmacol

Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization.

Authors - Hong Lu
humanWB,IHC,IF

J Pediatr Urol

Relationship of tumour-associated macrophages with poor prognosis in Wilms' tumour.

Authors - Kaixuan Tian