CD163 Polyclonal antibody
CD163 Polyclonal Antibody for FC, IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, pig and More (1)
Applications
WB, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 16646-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig spleen tissue, human placenta tissue |
| Positive IHC detected in | human tonsillitis tissue, human lung tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
| Positive FC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 10 publications below |
| IF | See 11 publications below |
| FC | See 1 publications below |
Product Information
The immunogen of 16646-1-AP is CD163 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CD163 fusion protein Ag10029 |
| Full Name | CD163 molecule |
| Calculated molecular weight | 1156 aa, 125 kDa |
| Observed molecular weight | 130-145 kDa |
| GenBank accession number | BC051281 |
| Gene symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD163 antibody 16646-1-AP | Download protocol |
| IHC protocol for CD163 antibody 16646-1-AP | Download protocol |
| IF protocol for CD163 antibody 16646-1-AP | Download protocol |
| FC protocol for CD163 antibody 16646-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Cancer Immunogenomic analysis reveals LGALS1 contributes to the immune heterogeneity and immunosuppression in glioma. | ||
J Ethnopharmacol The effect of Jianpi Yangzheng Xiaozheng Decoction and its components on gastric cancer. | ||
Am J Physiol Cell Physiol OSCC-derived exosomes promote M2-type macrophage polarization mediated by exosome-enclosed miR-29a-3p. | ||
EBioMedicine DNA damage repair alterations modulate M2 polarization of microglia to remodel the tumor microenvironment via the p53-mediated MDK expression in glioma. | ||
Biochem Pharmacol Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization. | ||
