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CD163 Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 16646-1-AP

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Con: 35 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, FC, ELISA
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Images See all

CD163 Antibody WB human placenta tissue 16646-1-AP

human placenta tissue were subjected to SDS PAGE followed by western blot with 16646-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

CD163 Antibody IHC human tonsillitis tissue 16646-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD163 Antibody IHC human tonsillitis tissue 16646-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 16646-1-AP (CD163 antibody) at dilution of 1:2400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD163 Antibody FC RAW 264.7 cells 16646-1-AP

1X10^6 RAW 264.7 cells were stained with 0.2ug CD163 antibody (16646-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse
Positive WB detected in:
human placenta tissue
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected in:
RAW 264.7 cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:1000-1:4800

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD163 fusion protein Ag10029
Full name:
CD163 molecule
Calculated molecular weight:
1156aa,125 kDa
Observed molecular weight:
145 kDa
GenBank accession number:
BC051281
Gene ID (NCBI):
9332
Gene symbol
CD163
Synonyms
CD163, CD163 molecule, Hemoglobin scavenger receptor, M130, MM130, sCD163

Background

CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.


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