Validation Data Gallery
|Positive WB detected in||pig spleen tissue, human placenta tissue|
|Positive IHC detected in||human tonsillitis tissue, human lung tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||RAW 264.7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 16646-1-AP is CD163 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, pig|
|Cited Reactivity||human, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD163 fusion protein Ag10029|
|Full Name||CD163 molecule|
|Calculated molecular weight||1156 aa, 125 kDa|
|Observed molecular weight||130-145 kDa|
|GenBank accession number||BC051281|
|Gene ID (NCBI)||9332|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD163 is a transmembrane protein which belongs to the scavenger receptor cysteine-rich (SRCR) superfamily. This protein is a scavenger receptor for the hemoglobin-haptoglobin complex and is a marker for monocytes and macrophages. Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity.
|Product Specific Protocols|
|WB protocol for CD163 antibody 16646-1-AP||Download protocol|
|IHC protocol for CD163 antibody 16646-1-AP||Download protocol|
|IF protocol for CD163 antibody 16646-1-AP||Download protocol|
|FC protocol for CD163 antibody 16646-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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