TEM1 Antibody

TEM1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 18160-1-AP

Synonyms

CD164L1, CD248, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1



Tested Applications

Positive WB detected inHeLa cells, A375 cells, COLO 320 cells, HEK-293 cells, mouse colon tissue, mouse heart tissue, mouse stomach tissue, NIH/3T3 cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue, human renal cell carcinoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18160-1-AP targets TEM1 in FC, IHC, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TEM1 fusion protein Ag12990
Full Name CD248 molecule, endosialin
Calculated molecular weight 757aa,81 kDa
Observed molecular weight 81 kDa
GenBank accession number BC051340
Gene symbol CD248
Gene ID (NCBI) 57124
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.

Protocols

Product Specific Protocols
WB protocol for TEM1 antibody 18160-1-APDownload protocol
IHC protocol for TEM1 antibody 18160-1-APDownload protocol
FC protocol for TEM1 antibody 18160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

BMC Cancer

TGFbeta-mediated suppression of CD248 in non-cancer cells via canonical Smad-dependent signaling pathways is uncoupled in cancer cells.

Authors - Sahana Suresh Babu
mouse, humanWB

PLoS One

Identification of a candidate proteomic signature to discriminate multipotent and non-multipotent stromal cells.

Authors - Rosu-Myles Michael M
humanWB,IHC

Oncotarget

Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma.

Authors - Emily Capone
mouseIF

EBioMedicine

Specific loss of adipocyte CD248 improves metabolic health via reduced white adipose tissue hypoxia, fibrosis and inflammation.

Authors - Paul Petrus