CD4 Polyclonal antibody

CD4 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Cat no : 11056-2-AP

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive WB detected inhuman spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

11056-2-AP targets CD4 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD4 fusion protein Ag0422
Full Name CD4 molecule
Calculated molecular weight 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Protocols

Product Specific Protocols
WB protocol for CD4 antibody 11056-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Biosci

Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression.

Authors - Anna Qin
humanIHC

J Physiol Biochem

α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.

Authors - Liping Wang

Acta Histochem

Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells.

Authors - Wenyan Gao
humanIHC

Viral Immunol

A Comparison of Splenic Pathologic Change and Immune Function in HBV-Related Portal Hypertension and Chinese Budd-Chiari Syndrome Patients with Hypersplenism.

Authors - Suxin Li
humanFC

DNA Cell Biol

Upregulated Expression of microRNA-16 Correlates with Th17/Treg Cell Imbalance in Patients with Rheumatoid Arthritis.

Authors - Yuan-Hao Wu
mouseWB,IF

Phytother Res

Casticin prevents DSS induced ulcerative colitis in mice through inhibitions of NF-κB pathway and ROS signaling.

Authors - Jiamei Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Luca (Verified Customer) (02-26-2018)

  • Applications: Other (please state below),
  • Primary Antibody Dilution: 10000 to 1 ng/ml