- Featured Product
- KD/KO Validated
CDK1-Specific Polyclonal antibody
CDK1-Specific Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 19532-1-AP
|Positive WB detected in||HEK-293 cells, MCF-7 cells, A431 cells, HeLa cells, HepG2 cells, C2C12 cells, C6 cells|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
19532-1-AP targets CDK1-Specific in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Full Name||cell division cycle 2, G1 to S and G2 to M|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||NM_001786|
|Gene ID (NCBI)||983|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. CDK1 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDK1 and function as regulatory subunits. CDK1 has 2 isoforms produced by alternative splicing with the molecular mass of 34 kDa and 27 kDa.
MiR-31-5p acts as a tumor suppressor in renal cell carcinoma by targeting cyclin-dependent kinase 1 (CDK1).
Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways.
Cordycepin suppresses cell proliferation and migration by targeting CLEC2 in human gastric cancer cells via Akt signaling pathway.
Onco Targets Ther
Prognostic implications and oncogenic roles of MYBL2 protein expression in esophageal squamous-cell carcinoma.
High expression of CDK1 and BUB1 predicts poor prognosis of pancreatic ductal adenocarcinoma.
Long noncoding RNA miR143HG, low expression in hepatocellular carcinoma, predicts a good prognosis and inhibits tumor multiplication and metastasis by suppressing MAPK and Wnt signaling pathways.