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CDK1-Specific Antibody 40 Publications

Rabbit Polyclonal| Catalog number: 19532-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, CoIP, ELISA
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Images See all

CDK1-Specific Antibody WB A431 cells 19532-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 19532-1-AP (CDK1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

CDK1-Specific Antibody IHC human cervical cancer tissue 19532-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP( CDK1-Specific antibody at dilution of 1:200 (under 10x lens).

CDK1-Specific Antibody IHC human cervical cancer tissue 19532-1-AP

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 19532-1-AP( CDK1-Specific antibody at dilution of 1:200 (under 40x lens).

CDK1-Specific Antibody IF HeLa cells 19532-1-AP

Immunofluorescent analysis of HeLa cells using 19532-1-AP(CDK1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected in:
human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
cell division cycle 2, G1 to S and G2 to M
Calculated molecular weight:
34 kDa
Observed molecular weight:
34 kDa
GenBank accession number:
NM_001786
Gene ID (NCBI):
983
Gene symbol
CDC2
Synonyms
CDC2, CDC28A, CDK1, Cell division protein kinase 1, Cyclin dependent kinase 1, p34 protein kinase

Background

CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily.CDC2 plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. It is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The antibody is specific to the 2 isoforms of CDC2.


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