CDC37 Antibody

CDC37 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF, WB, ELISA

Conjugate

Unconjugated

Publications

0

CloneNo.

1C2A6

Cat no : 66420-1-Ig

Synonyms

CDC37, CDC37A, Hsp90 co chaperone Cdc37, P50CDC37



Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:5000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66420-1-Ig targets CDC37 in IF, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CDC37 fusion protein Ag0267
Full Name cell division cycle 37 homolog (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight 44 kDa
GenBank accession number BC000083
Gene symbol CDC37
Gene ID (NCBI) 11140
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC(cell division control) family of proteins are regulatory proteins involved in the processes of cell cycle and proliferation. CDC37, migrated as a protein of 50 kDa in SDS PAGE, is a co-chaperone protein that recruits several immature client kinases to Hsp90 for proper folding. It functions in association with Hsp90, although little is known of its mechanism of action or where it fits into a folding pathway involving other Hsp90 cochaperones. CDC37 up-regulation is a common early event in some localized human cancers. But, the effect of CDC37 dysregulation in human cells is unclear.

Protocols

Product Specific Protocols
WB protocol for CDC37 antibody 66420-1-IgDownload protocol
IF protocol for CDC37 antibody 66420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols