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CDC42 Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 10155-1-AP

Featured Product KD/KO validated

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, CoIP, ELISA
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Images See all

CDC42 Antibody WB mouse brain tissue 10155-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10155-1-AP(CDC42 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

CDC42 Antibody IHC human pancreas cancer tissue 10155-1-AP

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10155-1-AP( CDC42 Antibody) at dilution of 1:200 (under 10x lens).

CDC42 Antibody IHC human pancreas cancer tissue 10155-1-AP

Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 10155-1-AP( CDC42 Antibody) at dilution of 1:200 (under 40x lens).

CDC42 Antibody WB C6 cells 10155-1-AP

C6 cells were subjected to SDS PAGE followed by western blot with 10155-1-AP( CDC42 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

CDC42 Antibody IF C6 cells 10155-1-AP

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed C6 cells using 10155-1-AP(CDC42 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, Jurkat cells, C6 cells, NIH/3T3 cells
Positive IHC detected in:
human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
C6 cells, HeLa cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:50-1:500
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CDC42 fusion protein Ag0207
Full name:
cell division cycle 42 (GTP binding protein, 25kDa)
Calculated molecular weight:
21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC002711
Gene ID (NCBI):
998
Gene symbol
CDC42
Synonyms
CDC42, CDC42Hs, G25K, G25K GTP binding protein

Background

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.


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