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  • KD/KO Validated

CDC42 Polyclonal antibody

CDC42 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10155-1-AP

Synonyms

CDC42, CDC42Hs, G25K, G25K GTP binding protein



Tested Applications

Positive WB detected inHT-29 cells, HeLa cells, C6 cells, Jurkat cells
Positive IHC detected inhuman pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inC6 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10155-1-AP targets CDC42 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC42 fusion protein Ag0207
Full Name cell division cycle 42 (GTP binding protein, 25kDa)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC002711
Gene symbol CDC42
Gene ID (NCBI) 998
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.

Protocols

Product Specific Protocols
WB protocol for CDC42 antibody 10155-1-APDownload protocol
IHC protocol for CDC42 antibody 10155-1-APDownload protocol
IF protocol for CDC42 antibody 10155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer.

Authors - Zitong Zhao
  • KD Validated
humanWB

Am J Transl Res

GRHL2 suppresses tumor metastasis via regulation of transcriptional activity of RhoG in non-small cell lung cancer.

Authors - Xiang Pan
humanWB

Oncotarget

Dihydroartemisinin suppresses pancreatic cancer cells via a microRNA-mRNA regulatory network.

Authors - Yilong Li

Int J Clin Exp Pathol

miR-29a inhibits adhesion, migration, and invasion of osteosarcoma cells by suppressing CDC42.

Authors - Zheng-Jie Liu
humanWB

Exp Ther Med

Effects of mevalonate kinase interference on cell differentiation, apoptosis, prenylation and geranylgeranylation of human keratinocytes are attenuated by farnesyl pyrophosphate or geranylgeranyl pyrophosphate.

Authors - Min Li
WB

Theriogenology

Influence of Wilms' tumor suppressor gene WT1 on bovine Sertoli cells polarity and tight junctions via non-canonical WNT signaling pathway.

Authors - Xue Wang