Tested Applications
| Positive WB detected in | HT-29 cells, C6 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 60 publications below |
| IHC | See 8 publications below |
| IF | See 8 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
10155-1-AP targets CDC42 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, bovine, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDC42 fusion protein Ag0207 Predict reactive species |
| Full Name | cell division cycle 42 (GTP binding protein, 25kDa) |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC002711 |
| Gene Symbol | CDC42 |
| Gene ID (NCBI) | 998 |
| RRID | AB_2078096 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60953 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC42 antibody 10155-1-AP | Download protocol |
| IHC protocol for CDC42 antibody 10155-1-AP | Download protocol |
| IF protocol for CDC42 antibody 10155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line. | ||
Circ. Res. Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm. | ||
Nucleic Acids Res DOCK7 protects against replication stress by promoting RPA stability on chromatin. | ||
Cell Mol Life Sci Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation | ||
Biomaterials Energy metabolism analysis reveals the mechanism of inhibition of breast cancer cell metastasis by PEG-modified graphene oxide nanosheets. | ||
Mol Ther Nucleic Acids miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (08-16-2023) | Worked well for WB and IF
|
FH Ambra (Verified Customer) (01-19-2021) | I used it 1:500 in 5% milk in TBS-tween to have a nice signal in Human Brain lysate.The signal is quite low used 1:1000.
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