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Cadherin-13 Polyclonal antibody

Cadherin-13 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12618-1-AP

Synonyms

Cadherin 13, Cadherin-13, CDH13, CDHH, H cadherin, H-cadherin, Heart cadherin, P105, T cad, T cadherin, T-cadherin, Truncated cadherin

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of mouse heart using 12618-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of L02 using 12618-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    WB analysis of human heart using 12618-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12618-1-AP (Cadherin-13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IP experiment of mouse heart using 12618-1-AP

    IP result of anti-Cadherin-13 (IP:12618-1-AP, 4ug; Detection:12618-1-AP 1:700) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human endometrial cancer using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12618-1-AP (Cadherin-13 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human heart using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using Cadherin-13 Polyclonal antibody (12618-1-AP)

    IHC staining of human heart using 12618-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12618-1-AP (Cadherin-13 antibody at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of Jurkat cells using Cadherin-13 Polyclonal antibody (12618-1-AP)

    FC experiment of Jurkat using 12618-1-AP

    1X10^6 Jurkat cells were stained with 0.5ug Cadherin-13 antibody (12618-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, L02 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman endometrial cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12618-1-AP targets Cadherin-13 in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cadherin-13 fusion protein Ag3295
Full Name cadherin 13, H-cadherin (heart)
Calculated Molecular Weight 713 aa, 78 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC030653
Gene Symbol CDH13
Gene ID (NCBI) 1012
RRIDAB_10644130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-13 (CDH13, also known as H-cadherin or T-cadherin) is an atypical member of cadherin family, lacking transmembrane and cytosolic domains and anchored to the exterior surface of the plasma membrane via a GPI anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is highly expressed in heart, and in the CNS, CDH13 is expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. It is often down-regulated in cancerous cells. The CDH13 cDNA encodes a 713-amino acid protein, which has a calculated molecular weight of 78 kDa. Studies have detected variable bands on Western blots, including 105 kDa (mature form), 130 kDa (precursor), and 45 kDa, which may be a post-translational processing variant of CDH13 (PMID: 19399994).

Protocols

Product Specific Protocols
WB protocol for Cadherin-13 antibody 12618-1-APDownload protocol
IHC protocol for Cadherin-13 antibody 12618-1-APDownload protocol
IP protocol for Cadherin-13 antibody 12618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols