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- KD/KO Validated
CDK2 Polyclonal antibody
CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 10122-1-AP
|Positive WB detected in||HEK-293 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, HL-60 cells, Jurkat cells, A431 cells, rat kidney tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human liver cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sea cucumbers, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDK2 fusion protein Ag0166|
|Full Name||cyclin-dependent kinase 2|
|Calculated molecular weight||33 kDa|
|Observed molecular weight||33 kDa|
|GenBank accession number||BC003065|
|Gene ID (NCBI)||1017|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IHC protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IF protocol for CDK2 antibody 10122-1-AP||Download protocol|
|IP protocol for CDK2 antibody 10122-1-AP||Download protocol|
|FC protocol for CDK2 antibody 10122-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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