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KD/KO Validated

CDK2 Antibody

Name: CDK2 Rabbit Polyclonal antibody
Brand: Proteintech
SKU: 10122-1-AP

CDK2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10122-1-AP

Synonyms

CDK2, Cell division protein kinase 2, cyclin dependent kinase 2, p33 protein kinase, p33(CDK2)

Validation Data Gallery

IHC staining of human liver cancer tissue using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
IHC staining of human liver cancer tissue using 10122-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10122-1-AP (CDK2 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
IF staining of HepG2 cells using 10122-1-AP
Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 10122-1-AP (CDK2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
WB analysis of HEK-293 cells using 10122-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours
IP experiment of HeLa cells using 10122-1-AP
IP Result of anti-CDK2 (IP:10122-1-AP, 3ug; Detection:10122-1-AP 1:1500) with HeLa cells lysate 4650ug.
FC experiment of HepG2 cells using 10122-1-AP
1X10^6 HepG2 cells were stained with 0.2ug CDK2 antibody (10122-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Cat No. 10122-1-AP

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Tested Applications

Positive WB detected in	HEK-293 cells, rat kidney tissue, K-562 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, HL-60 cells, Jurkat cells, A431 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 84 publications below
IHC	See 3 publications below
CoIP	See 1 publications below

Product Information

10122-1-AP targets CDK2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, sea cucumbers, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CDK2 fusion protein Ag0166
Full Name	cyclin-dependent kinase 2
Calculated molecular weight	33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC003065
Gene symbol	CDK2
Gene ID (NCBI)	1017
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CDK2 antibody 10122-1-AP	Download protocol
IHC protocol for CDK2 antibody 10122-1-AP	Download protocol
IF protocol for CDK2 antibody 10122-1-AP	Download protocol
IP protocol for CDK2 antibody 10122-1-AP	Download protocol
FC protocol for CDK2 antibody 10122-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancers (Basel) Antrocin Sensitizes Prostate Cancer Cells to Radiotherapy through Inhibiting PI3K/AKT and MAPK Signaling Pathways. Authors - Yu-An Chen View Article
human	WB	Biomed Pharmacother Lin28 promotes dental pulp cell proliferation via upregulation of cyclin-dependent proteins and interaction with let-7a/IGF2BP2 pathways. Authors - Yan Liu View Article
human	WB	Biosci Rep The selective HDAC6 inhibitor Nexturastat A induces apoptosis, overcomes drug resistance and inhibits tumor growth in multiple myeloma. Authors - Xiaoyang Sun View Article
human	WB	BMC Cancer Short-term stimulation with histone deacetylase inhibitor trichostatin a induces epithelial-mesenchymal transition in nasopharyngeal carcinoma cells without increasing cell invasion ability. Authors - Zhihua Shen View Article
human	WB	J Enzyme Inhib Med Chem Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma. Authors - Feifei Qin View Article
human	WB	Biochem Pharmacol Inhibition of Wee1 sensitizes AML cells to ATR inhibitor VE-822-induced DNA damage and apoptosis. Authors - Wenxiu Qi View Article