A431 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Various lysates were subjected to SDS PAGE followed by western blot with 10122-1-AP (CDK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

HL-60 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

K-562 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

MCF7 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

rat kidney tissue were subjected to SDS PAGE followed by western blot with 10122-1-AP(CDK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

IP Result of anti-CDK2 (IP:10122-1-AP, 3ug; Detection:10122-1-AP 1:1500) with HeLa cells lysate 4650ug.

Immunofluorescent analysis of HepG2 cells, using CDK2 antibody 10122-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HepG2 cells were stained with 0.2ug CDK2 antibody (10122-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.