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  • Featured Product
  • KD/KO Validated

CDK5RAP3 Polyclonal antibody

CDK5RAP3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11007-1-AP

Synonyms

C53, CDK5RAP3, HSF 27, IC53, LZAP, MST016, OK/SW cl.114, PP1553, Protein HSF 27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    WB analysis using 11007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    WB analysis of HEK-293 using 11007-1-AP

    WB result of CDK5RAP3 antibody (11007-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK5RAP3 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    WB analysis of HeLa using 11007-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    WB analysis of A431 using 11007-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human stomach using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IHC staining of human pancreas using 11007-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IF Staining of human liver using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CDK5RAP3 antibody (11007-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IF Staining of HepG2 using 11007-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11007-1-AP (CDK5RAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    IF Staining of HepG2 using 11007-1-AP

    Immunofluorescent analysis of HepG2 cells, using CDK5RAP3 antibody 11007-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Flow cytometry (FC) experiment of HepG2 cells using CDK5RAP3 Polyclonal antibody (11007-1-AP)

    FC experiment of HepG2 using 11007-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CDK5RAP3 antibody (11007-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HEK-293 cells, HEK-293T cells, Jurkat cells
Positive IHC detected inhuman stomach tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, human liver tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11007-1-AP targets CDK5RAP3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK5RAP3 fusion protein Ag1474
Full Name CDK5 regulatory subunit associated protein 3
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC009957
Gene Symbol CDK5RAP3
Gene ID (NCBI) 80279
RRIDAB_2076869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin-dependent kinase 5 (CDK5) regulatory subunit associated protein 3 (CDK5RAP3, also named as C53 or LZAP) was initially identified as a binding protein of CDK5 activator p35. CDK5RAP3 is widely expressed in human tissues and broadly located in subcellular compartments. By interacting with other proteins, CDK5RAP3 participates in the regulation of multiple cellular processes including cell cycle, apoptosis, cell invasion, signaling transduction, autophagy, UFMylation and endoplasmic reticulum (ER) stress.

Protocols

Product Specific Protocols
WB protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IHC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
IF protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
FC protocol for CDK5RAP3 antibody 11007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,bovineWB

Int J Mol Sci

CDK5RAP3, a Novel Nucleoplasmic Shuttle, Deeply Regulates HSF1-Mediated Heat Stress Response and Protects Mammary Epithelial Cells from Heat Injury.

Authors - Yangyang Shen
  • KD Validated
View Article