Tested Applications
| Positive WB detected in | Jurkat cells, A549 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23431-1-AP targets CEBPB in WB, IHC, IF/ICC, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, chicken, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20073 Product name: Recombinant human CEBPB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-266 aa of BC007538 Sequence: MQRLVAWDPACLPLPPPPPAFKSMEVANFYYEADCLAAAYGGKAAPAAPPAARPGPRPPAGELGSIGDHERAIDFSPYLEPLGAPQAPAPATATDTFEAAPPAPAPAPASSGQHHDFLSDLFSDDYGGKNCKKPAEYGYVSLGRLGAAKGALHPGCFAPLHPPPPPPPPPAELKAEPGFEPADCKRKEEAGAPGGGAGMAAGFPYALRAYLGYQAVPSGSSGSLSTSSSSSPPGTPSPADAKAPPTACYAGAAPAPSQVKSKAKKT Predict reactive species |
| Full Name | CCAAT/enhancer binding protein (C/EBP), beta |
| Calculated Molecular Weight | 345 aa, 36 kDa |
| Observed Molecular Weight | 42 kDa,46 kDa |
| GenBank Accession Number | BC007538 |
| Gene Symbol | CEBPB |
| Gene ID (NCBI) | 1051 |
| RRID | AB_2879278 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17676 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298). This antibody is specific to CEBPB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CEBPB antibody 23431-1-AP | Download protocol |
| IHC protocol for CEBPB antibody 23431-1-AP | Download protocol |
| WB protocol for CEBPB antibody 23431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C/EBPβ antibody (Cat# 23431-1-AP) has accumulated 79 citations across journals including Cancer Cell, Nature Genetics, Nature Immunology, Nature Communications, Nature Metabolism, Advanced Science, Cell Reports, Cell Death & Differentiation, FASEB Journal, and many others. Research fields span oncology (colorectal, esophageal, breast, glioma, renal cell carcinoma), cardiology, immunology, lipid/glucose metabolism, neurology, inflammation, developmental biology, and virology. Applications include WB, ChIP, CoIP, IHC, IF, and RIP.
| Species | Application | Title |
|---|---|---|
Cancer Cell Epithelial cells activate fibroblasts to promote esophageal cancer development | ||
Nat Metab Proline uptake promotes activation of lymphoid tissue inducer cells to maintain gut homeostasis | ||
Nat Genet Single-nucleus multi-omic profiling of human placental syncytiotrophoblasts identifies cellular trajectories during pregnancy | ||
Clin Mol Hepatol CD36 Promotes Iron Accumulation and Dysfunction in CD8+ T Cells via the p38-CEBPB-TfR1 Axis in Early-stage Hepatocellular Carcinoma | ||
Nat Commun Intercellular chemerin-cmklr1 couples epicardial mechanosensing to fibroblasts in pressure overload. |
