CEP97 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 22050-1-AP

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Con: 50 μg/150 μl

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Species specificity:
human, dog

Positive WB detected in:
HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells

Positive IP detected in:
HeLa cells

Positive IHC detected in:
human breast cancer tissue, human breast cancer tissue, human liver tissue

Positive IF detected in:
MDCK cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
centrosomal protein 97kDa

Calculated molecular weight:
865aa,97 kDa

Observed molecular weight:
130-140 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CEP97

Synonyms
2810403B08Rik, centrosomal protein 97kDa, Centrosomal protein of 97 kDa, CEP97, FLJ23047, FLJ26462, LRRIQ2
Background

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.


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