|Positive WB detected in||HL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22050-1-AP is CEP97 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, Canine|
|Cited Reactivity||Drosophila, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CEP97 fusion protein Ag16995|
|Full Name||centrosomal protein 97kDa|
|Calculated molecular weight||865 aa, 97 kDa|
|Observed molecular weight||130-140 kDa|
|GenBank accession number||BC041085|
|Gene ID (NCBI)||79598|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.
|Product Specific Protocols|
|WB protocol for CEP97 antibody 22050-1-AP||Download protocol|
|IHC protocol for CEP97 antibody 22050-1-AP||Download protocol|
|IF protocol for CEP97 antibody 22050-1-AP||Download protocol|
|IP protocol for CEP97 antibody 22050-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.
CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.
Hum Mol Genet
Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.
LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.
A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
J Cell Biol
Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.