CEP97 Polyclonal antibody

CEP97 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, dog and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22050-1-AP

Synonyms

2810403B08Rik, centrosomal protein 97kDa, Centrosomal protein of 97 kDa, CEP97, LRRIQ2



Tested Applications

Positive WB detected inHL-60 cells, HEK-293 cells, HeLa cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22050-1-AP is CEP97 Fusion Protein expressed in E. coli.

Tested Reactivity human, dog
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP97 fusion protein Ag16995
Full Name centrosomal protein 97kDa
Calculated molecular weight 865 aa, 97 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC041085
Gene symbol CEP97
Gene ID (NCBI) 79598
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

Protocols

Product Specific Protocols
WB protocol for CEP97 antibody 22050-1-APDownload protocol
IHC protocol for CEP97 antibody 22050-1-APDownload protocol
IF protocol for CEP97 antibody 22050-1-APDownload protocol
IP protocol for CEP97 antibody 22050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Protein Cell

Neddylation inhibitor MLN4924 suppresses cilia formation by modulating AKT1.

Authors - Hongmei Mao
humanIF

Cell Rep

CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation.

Authors - Tianning Wang
humanIF

Hum Mol Genet

Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.

Authors - Mairéad Flynn
humanWB,IP

Elife

LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.

Authors - Laura Bozal-Basterra
human

Cell Rep

A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.

Authors - Kari-Anne M Frikstad
humanIF

J Cell Biol

Phosphorylation of CEP83 by TTBK2 is necessary for cilia initiation.

Authors - Chien-Hui Lo