Cingulin Polyclonal antibody

Cingulin Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21369-1-AP


CGN, Cingulin, KIAA1319

Tested Applications

Positive WB detected inHEK-293T cells, mouse kidney tissue, mouse small intestine tissue, MCF-7 cells
Positive IP detected inmouse kidney tissue

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21369-1-AP is Cingulin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cingulin fusion protein Ag15843
Full Name cingulin
Calculated molecular weight 1197 aa, 136 kDa
Observed molecular weight 136-150 kDa
GenBank accession numberBC146657
Gene symbol CGN
Gene ID (NCBI) 57530
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.


Product Specific Protocols
WB protocol for Cingulin antibody 21369-1-APDownload protocol
IP protocol for Cingulin antibody 21369-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


mouseWB, IP

Cell Signal

Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.

Authors - Serge Ducommun

Nat Commun

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Authors - Yi Liu