CHAF1A Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 17037-1-AP

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Freight/Packing

Con: 41 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
Jurkat cells, HeLa cells, Jurkat cells

Positive IP detected in:
Jurkat cells

Positive IHC detected in:
human cervix tissue

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:1000-1:4000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
chromatin assembly factor 1, subunit A (p150)

Calculated molecular weight:
956aa,107 kDa

Observed molecular weight:
150 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CHAF1A

Synonyms
CAF, CAF 1, CAF 1 subunit A, CAF I 150 kDa subunit, CAF I p150, CAF1, CAF1B, CAF1P150, CHAF1A, hp150, P150
Background

Chromatin assembly factor 1 (CAF1) is the only histone chaperone known to assemble histones H3 and H4 onto newly synthesized DNA both in vitro and in vivo [PMID:17065558]. The 938 amino acid multidomain p150 (CHAF1A) binds via its C-terminal third to p60, which is an essential step for nucleosome assembly because knocking down either subunit disrupts the activity [PMID:14519857]. In addition, CAF1 facilitates DNA synthesis depending on the binding of the N-terminal 31 residues of p150 to the proliferating cell nuclear antigen (PCNA), which acts as a sliding clamp to stimulate the processivity of DNA polymerase [PMID:10648606]. CHAF1A regulates the formation of heterochromatin in mammalian cells during replication and in plants it maintains the transcription of certain subsets of genes. Furthermore, CHAF1A exists in a chromatin-remodeling complex WINAC, which coactivates ligand-induced transactivation function of the vitamin D receptor [PMID:12837248].


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