CHAT Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 20747-1-AP

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Freight/Packing

Con: 59 μg/150 μl

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Species specificity:
human, mouse, rat

Positive WB detected in:
rat brain tissue, human placenta tissue, Jurkat cells, mouse placenta tissue

Positive IP detected in:
rat brain tissue

Positive IHC detected in:
human brain tissue,human lung cancer tissue, rat brain tissue, mouse brain tissue

Positive IF detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
choline acetyltransferase

Calculated molecular weight:
83 kDa

Observed molecular weight:
47-50, 66-70 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
CHAT

Synonyms
CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2
Background

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively. This antibody mainly recognizes the 45-50 kDa pCHAT in multiple lysates.


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