Chk2 Polyclonal antibody

Chk2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13954-1-AP

Synonyms

CDS1, CHEK2, Chk2, HuCds1, LFS2, PP1425, RAD53



Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHL-60 cells
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 11 publications below

Product Information

13954-1-AP targets Chk2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Chk2 fusion protein Ag5065
Full Name CHK2 checkpoint homolog (S. pombe)
Calculated molecular weight 61 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC004207
Gene symbol CHEK2
Gene ID (NCBI) 11200
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chk2(Checkpoint kinase 2) is also named as CDS1, CHEK2, RAD53 and belongs to the CHK2 subfamily. It is a protein kinase that is activated in response to DNA damage and involved in cell cycle arrest. It also mediates the induction of senescence in fibroblasts, but is dispensable for the induction of telomere dysfunction checkpoints at the stem and progenitor cell level(PMID:20577265). This protein can exsit as a dimer (PMID:19782031). Active forms of CHEK2 devoid of phosphorylation at Thr-68 can form unstable dimers(PMID:12386164). Defects in CHEK2 is a cause of susceptibility to breast cancer (BC)(PMID:21618645). It has 12 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for Chk2 antibody 13954-1-APDownload protocol
IF protocol for Chk2 antibody 13954-1-APDownload protocol
IP protocol for Chk2 antibody 13954-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biometals

Probing the cell death signaling pathway of HepG2 cell line induced by copper-1,10-phenanthroline complex.

Authors - Jieyuan Wu
ratWB

Int J Nanomedicine

TiO2 Nanoparticles Caused DNA Damage in Lung and Extra-Pulmonary Organs Through ROS-Activated FOXO3a Signaling Pathway After Intratracheal Administration in Rats.

Authors - Bin Han
ratWB

J Sci Food Agric

Rice protein depresses DNA damage through activating p53 pathway and stimulating endogenous antioxidant response in growing and adult rats.

Authors - Mingcai Liang
mouseWB

Plant Foods Hum Nutr

Anthocyanins Delay Ageing-Related Degenerative Changes in the Liver.

Authors - Jie Wei
mouseWB

J Agric Food Chem

Anthocyanins from Black Chokeberry (Aroniamelanocarpa Elliot) Delayed Aging-Related Degenerative Changes of Brain.

Authors - Jie Wei
humanWB

Med Oncol

PMID: 31372848

Authors - Jingrui Jin