|Positive WB detected in||mouse heart tissue, transfected cells|
|Positive IHC detected in||human oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13683-1-AP targets CHMP4B in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHMP4B fusion protein Ag4544|
|Full Name||chromatin modifying protein 4B|
|Calculated molecular weight||224 aa, 25 kDa|
|Observed molecular weight||29-33 kDa|
|GenBank accession number||BC033859|
|Gene ID (NCBI)||128866|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn’t cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).
Vps4A mediates the localization and exosome release of β-catenin to inhibit epithelial-mesenchymal transition in hepatocellular carcinoma.
Methods Mol Biol
Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.
Cell Death Dis
Restoration of lysosomal function after damage is accompanied by recycling of lysosomal membrane proteins.
Synthetic Lethal Interaction between the ESCRT Paralog Enzymes VPS4A and VPS4B in Cancers Harboring Loss of Chromosome 18q or 16q.
Human Cytomegalovirus Utilizes Extracellular Vesicles to Enhance Virus Spread.
A non-envelopment role for the ESCRT-III complex during HCMV infection.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sharad (Verified Customer) (08-30-2019)
The antibody works well in IF staining 1:500 dilution.