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CHMP4B Antibody 9 Publications

Rabbit Polyclonal| Catalog number: 13683-1-AP

Featured Product KD/KO validated

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CHMP4B Antibody WB mouse heart tissue 13683-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP(CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

CHMP4B Antibody WB transfected cells 13683-1-AP

WB result of anti-CHMP4B (13683-1-AP) with “in vitro translated MYC-CHMP4B in wheatgerm lysates”.

CHMP4B Antibody IHC human oesophagus cancer tissue 13683-1-AP

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP(CHMP4B antibody) at dilution of 1:50 (under 10x lens)

CHMP4B Antibody IHC human oesophagus cancer tissue 13683-1-AP

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP(CHMP4B antibody) at dilution of 1:50 (under 40x lens)

CHMP4B Antibody IF NIH/3T3 cells 13683-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, transfected cells
Positive IHC detected in:
human oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CHMP4B fusion protein Ag4544
Full name:
chromatin modifying protein 4B
Calculated molecular weight:
224aa,25 kDa
Observed molecular weight:
29-33 kDa
GenBank accession number:
BC033859
Gene ID (NCBI):
128866
Gene symbol
CHMP4B
Synonyms
C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

Background

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn’t cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).


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