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CHMP4B Antibody 10 Publications

Rabbit Polyclonal| Catalog number: 13683-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

CHMP4B Antibody WB transfected cells 13683-1-AP

WB result of anti-CHMP4B (13683-1-AP) with “in vitro translated MYC-CHMP4B in wheatgerm lysates”.

CHMP4B Antibody WB mouse heart tissue 13683-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP(CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours

CHMP4B Antibody IHC human oesophagus cancer tissue 13683-1-AP

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP(CHMP4B antibody) at dilution of 1:50 (under 10x lens)

CHMP4B Antibody IHC human oesophagus cancer tissue 13683-1-AP

Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP(CHMP4B antibody) at dilution of 1:50 (under 40x lens)

CHMP4B Antibody IF NIH/3T3 cells 13683-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse heart tissue, transfected cells
Positive IHC detected in:
human oesophagus cancer tissue, human heart tissue, human skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
NIH/3T3 cells
Recommended dilution:
WB : 1:200-1:1000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CHMP4B fusion protein Ag4544
Full name:
chromatin modifying protein 4B
Calculated molecular weight:
224aa,25 kDa
Observed molecular weight:
29-33 kDa
GenBank accession number:
BC033859
Gene ID (NCBI):
128866
Gene symbol
CHMP4B
Synonyms
C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

Background

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn’t cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

CHMP4B Rabbit Polyclonal antibody

Sharad (Verified Customer), 08-30-2019
Applications
Immunofluorescence,
No image submitted with review

No image submitted with review

Cell/Tissue Type
RPE-1
Lot Number
00022664
The antibody works well in IF staining 1:500 dilution.

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