Tested Applications
| Positive WB detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21586-1-AP targets CHRNA7/CHRFAM7A in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16151 Product name: Recombinant human CHRFAM7A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-386 aa of BC101348 Sequence: TRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFS Predict reactive species |
| Full Name | CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion |
| Calculated Molecular Weight | 412 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC101348 |
| Gene Symbol | CHRNA7/CHRFAM7A |
| Gene ID (NCBI) | 89832 |
| RRID | AB_10734326 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q494W8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHRNA7/CHRFAM7A antibody 21586-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
