|Positive WB detected in||Raji cells, fetal human brain tissue, NIH/3T3 cells, THP-1 cells, Jurkat cells, Ramos cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Sample-dependent, check data in validation data gallery|
12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CIN85 fusion protein Ag2777|
|Full Name||SH3-domain kinase binding protein 1|
|Calculated molecular weight||665 aa, 73 kDa|
|Observed molecular weight||85 kDa|
|GenBank accession number||BC015806|
|Gene ID (NCBI)||30011|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.
Arch Gynecol Obstet
Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.
ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.
J Cell Biol
CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.