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CIN85 Antibody

CIN85 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12132-1-AP

Synonyms

CD2 binding protein 3, CD2BP3, CIN85, GIG10, HSB 1, MIG18, SH3KBP1



Tested Applications

Positive WB detected inRaji cells, fetal human brain tissue, NIH/3T3 cells, THP-1 cells, Jurkat cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

12132-1-AP targets CIN85 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIN85 fusion protein Ag2777
Full Name SH3-domain kinase binding protein 1
Calculated molecular weight 665aa,73 kDa
Observed molecular weight 85 kDa
GenBank accession number BC015806
Gene symbol SH3KBP1
Gene ID (NCBI) 30011
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Protocols

Product Specific Protocols
WB protocol for CIN85 antibody 12132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Arch Gynecol Obstet

Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.

Authors - Ma Yaxi Y
humanWB

Biol Cell

ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.

Authors - Yoon Hye-Young HY
  • KD Validated
humanWB, IF

J Cell Biol

CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.

Authors - Ihor Yakymovych