Claudin 18 Polyclonal antibody

Claudin 18 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21126-1-AP

Synonyms

claudin 18, CLDN18, SFTA5, SFTPJ



Tested Applications

Positive WB detected inmouse kidney tissue
Positive IP detected inmouse stomach tissue
Positive IHC detected inhuman stomach tissue, human ovary tumor tissue, human pancreas cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMKN-45 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21126-1-AP is Claudin 18 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 18 fusion protein Ag15517
Full Name claudin 18
Calculated molecular weight 261 aa, 28 kDa
Observed molecular weight 28-29 kDa
GenBank accession numberBC146668
Gene symbol CLDN18
Gene ID (NCBI) 51208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145).

Protocols

Product Specific Protocols
WB protocol for Claudin 18 antibody 21126-1-APDownload protocol
IHC protocol for Claudin 18 antibody 21126-1-APDownload protocol
IF protocol for Claudin 18 antibody 21126-1-APDownload protocol
IP protocol for Claudin 18 antibody 21126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

J Stroke Cerebrovasc Dis

Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.

Authors - Chunyan Wang
mouseWB,IF

Int J Mol Med

Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.

Authors - Ruixue Yang