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  • Featured Product
  • KD/KO Validated

CLIC4 Polyclonal antibody

CLIC4 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12298-2-AP

Synonyms

CLIC4, CLIC4L, H1, huH1, MTCLIC, p64H1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CLIC4 Polyclonal antibody (12298-2-AP)

    WB analysis of HeLa using 12298-2-AP

    WB result of CLIC4 antibody (12298-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse kidney tissue using CLIC4 Polyclonal antibody (12298-2-AP)

    WB analysis of mouse kidney using 12298-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse ovary tissue using CLIC4 Polyclonal antibody (12298-2-AP)

    WB analysis of mouse ovary using 12298-2-AP

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using CLIC4 Polyclonal antibody (12298-2-AP)

    WB analysis of human kidney using 12298-2-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12298-2-AP (CLIC4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Polyclonal antibody (12298-2-AP)

    IHC staining of human kidney using 12298-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CLIC4 Polyclonal antibody (12298-2-AP)

    IHC staining of human kidney using 12298-2-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12298-2-AP (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue, HeLa cells, mouse ovary tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12298-2-AP targets CLIC4 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIC4 fusion protein Ag2942
Full Name chloride intracellular channel 4
Calculated Molecular Weight 253 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC012444
Gene Symbol CLIC4
Gene ID (NCBI) 25932
RRIDAB_2081969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Protocols

Product Specific Protocols
WB protocol for CLIC4 antibody 12298-2-APDownload protocol
IHC protocol for CLIC4 antibody 12298-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols