beta Actin Fusion Protein

Catalog number: 66009-1-Ig
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99.0000

Freight/Packing £00.00

Size: 09µ/gram

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Product Name:
beta Actin Mouse Monoclonal antibody

Catalog No:
66009-1-Ig

Source:
E. coli-derived, {{ptg:Vector}}

Peptide Sequence:
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({{ptg:AntigenPeptide}} encoded by NM_001101)

Activity:
Not tested.

Endotoxin Level:
Please contact the lab for more information.
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Formulation:
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Reconstitution:
Reconstitute at {{ptg:SizeDesc}} sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.

Shipping:
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Stability and Storage:
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Storage of Reconstituted Protein:
Short-term storage: Store at 2-8°C for two weeks.
Long-term storage: Aliquot and store at -20°C to -80°C for up to 6 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b.


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