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beta Actin Antibody 349 Publications

Mouse Monoclonal| Catalog number: 66009-1-Ig |CloneNo.: 2D4H5

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Con: 64 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

beta Actin Antibody WB A549 cells 66009-1-Ig

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

beta Actin Antibody WB multi-cells 66009-1-Ig

Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds)

beta Actin Antibody WB HeLa cells 66009-1-Ig

WB result of anti-beta Actin monoclonal antibody (66009-1-Ig) with HeLa lysate at various dilutions.

beta Actin Antibody IF HepG2 cells 66009-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66009-1-Ig(beta Actin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

beta Actin Antibody IP HeLa cells 66009-1-Ig

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

beta Actin Antibody FC HeLa cells 66009-1-Ig

1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (66009-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

beta Actin Antibody IHC human kidney tissue 66009-1-Ig

Immunohistochemical of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens)

Species specificity:
human, mouse, rat, hamster, zebrafish, monkey, chicken, Drosophila, goat, pig, swine
Positive WB detected in:
multi-cells, HeLa cells, CHO cells, A549 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
actin, beta
Calculated molecular weight:
42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used “housekeeping gene” in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer’s disease (PMID: 11246152).



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