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beta Actin Antibody 515 Publications

Mouse Monoclonal| Catalog number: 66009-1-Ig |CloneNo.: 2D4H5

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3 customer reviews

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Con: 64 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

beta Actin Antibody WB multi-cells 66009-1-Ig

Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds)

beta Actin Antibody IF MDCK cells 66009-1-Ig

Immunofluorescent analysis of ( -20°C Methanol) fixed MDCK cells using 66009-1-Ig (beta Actin antibody) at dilution of 1:1000 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

beta Actin Antibody WB A549 cells 66009-1-Ig

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

beta Actin Antibody IP HeLa cells 66009-1-Ig

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

beta Actin Antibody FC HeLa cells 66009-1-Ig

1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (66009-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

beta Actin Antibody IHC human kidney tissue 66009-1-Ig

Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens)

Species specificity:
human, mouse, rat, hamster, zebrafish, monkey, chicken, chicks, cow, Drosophila, goat, grouper, pig
Positive WB detected in:
multi-cells, A549 cells, CHO cells, HeLa cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MDCK cells, HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:200-1:1500
Sample-dependent, check data in Validation tab

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
actin, beta
Calculated molecular weight:
42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used “housekeeping gene” in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer’s disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

beta Actin Mouse Monoclonal antibody

Huai-Chin (Verified Customer), 06-19-2019
Applications
Western Blot,
Primary Antibody Dilution
1:3000
No image submitted with review

No image submitted with review

Cell/Tissue Type
Primary cells, various cell lines
Lot Number
Very good anitbody. Works well in Western for various type of cells.

beta Actin Mouse Monoclonal antibody

Francesco (Verified Customer), 06-12-2019
Applications
Immunohistochemistry,
Primary Antibody Dilution
1/1500
No image submitted with review

No image submitted with review

Cell/Tissue Type
Brain cells
Lot Number
in the testing staining it kind of worked, even if with higher dilution it could work better. anyway further test should be done to confirm it. if results are reproducible it might be a valid alternative to more expensive antibody

beta Actin Mouse Monoclonal antibody

Kyosuke (Verified Customer), 06-05-2019
Applications
Western Blot,
Primary Antibody Dilution
1/1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
aorta
Lot Number
This antibody works very well for Western blot using vascular tissue or cell lysates.

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