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beta Actin Antibody 322 Publications

Mouse Monoclonal| Catalog number: 66009-1-Ig |CloneNo.: 2D4H5

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Con: 64 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

beta Actin Antibody WB multi-cells 66009-1-Ig

Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody 66009-1-Ig at a dilution of 1:20000. (Exposure time: 10 seconds)

beta Actin Antibody WB A549 cells 66009-1-Ig

A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000.

beta Actin Antibody WB HeLa cells 66009-1-Ig

WB result of anti-beta Actin monoclonal antibody (66009-1-Ig) with HeLa lysate at various dilutions.

beta Actin Antibody IF HepG2 cells 66009-1-Ig

Immunofluorescent analysis of ( -20℃ Ethanol ) fixed HepG2 cells using 66009-1-Ig(beta Actin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

beta Actin Antibody IP HeLa cells 66009-1-Ig

IP Result of anti-beta actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug.

beta Actin Antibody FC HeLa cells 66009-1-Ig

1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (66009-1-Ig, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

beta Actin Antibody IHC human kidney tissue 66009-1-Ig

Immunohistochemical of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens)

Species specificity:
human, mouse, rat, hamster, zebrafish, monkey, chicken, Drosophila, goat, pig, swine
Positive WB detected in:
multi-cells, HeLa cells, CHO cells, A549 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue
Positive IF detected in:
HepG2 cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:20-1:2000
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein A purification
Isotype:
IgG2b
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Fusion Protein
Full name:
actin, beta
Calculated molecular weight:
42 kDa
Observed molecular weight:
42 kDa
GenBank accession number:
NM_001101
Gene ID (NCBI):
60
Gene symbol
ACTB
Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

Background

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b.


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