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  • KD/KO Validated

CLIC1 Polyclonal antibody

CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14545-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

G6, NCC27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human colon tissue, human pancreas tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14545-1-AP targets CLIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIC1 fusion protein Ag6049 Predict reactive species
Full Name chloride intracellular channel 1
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27-32 kDa
GenBank Accession NumberBC064527
Gene Symbol CLIC1
Gene ID (NCBI) 1192
RRIDAB_2081809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CLIC1 antibody 14545-1-APDownload protocol
IHC protocol for CLIC1 antibody 14545-1-APDownload protocol
IF protocol for CLIC1 antibody 14545-1-APDownload protocol
IP protocol for CLIC1 antibody 14545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Alzheimers Res Ther

Circular RNA APP contributes to Alzheimer's disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis

Authors - Deng-Pan Wu
ratIF

Oxid Med Cell Longev

Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.

Authors - Songhan Li
humanWB

Transl Oncol

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling

Authors - Huiwu Geng
  • KD Validated

Cell Biochem Biophys

CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.

Authors - Dezhao Lu
humanIHC

Anticancer Res

Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.

Authors - Yuichiro Yasuda
mouseWB,IHC

Technol Cancer Res Treat

CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.

Authors - Xuyong Wei
  • KO Validated
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