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Ezrin Polyclonal antibody
Ezrin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, RIP, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 26056-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, mouse kidney tissue, NIH/3T3 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse stomach tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 10 publications below |
IF | See 2 publications below |
RIP | See 1 publications below |
Product Information
26056-1-AP targets Ezrin in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Ezrin fusion protein Ag23530 |
Full Name | ezrin |
Calculated molecular weight | 69 kDa |
Observed molecular weight | 70-75 kDa |
GenBank accession number | BC013903 |
Gene symbol | EZR |
Gene ID (NCBI) | 7430 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Ezrin antibody 26056-1-AP | Download protocol |
IHC protocol for Ezrin antibody 26056-1-AP | Download protocol |
IF protocol for Ezrin antibody 26056-1-AP | Download protocol |
IP protocol for Ezrin antibody 26056-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Neuropathol Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis. | ||
Proc Natl Acad Sci U S A Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.
| ||
Elife Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility. | ||
J Biol Chem Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments. | ||
Sci Rep Quantitative Proteomic analysis on Activated Hepatic Stellate Cells reversion Reveal STAT1 as a key regulator between Liver Fibrosis and recovery. |