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  • KD/KO Validated

Ezrin Polyclonal antibody

Ezrin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26056-1-AP

Synonyms

CVIL, CVL, Cytovillin, EZR, Ezrin, p81, VIL2, Villin 2



Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse stomach tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26056-1-AP is Ezrin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ezrin fusion protein Ag23530
Full Name ezrin
Calculated molecular weight 69 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC013903
Gene symbol EZR
Gene ID (NCBI) 7430
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Ezrin antibody 26056-1-APDownload protocol
IHC protocol for Ezrin antibody 26056-1-APDownload protocol
IF protocol for Ezrin antibody 26056-1-APDownload protocol
IP protocol for Ezrin antibody 26056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Ther Med

Role of mTOR signaling in the regulation of high glucose-induced podocyte injury.

Authors - Qiuyue Li
humanWB

Int J Mol Sci

Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.

Authors - Krishnendu Khan
  • KD Validated
mouseWB

J Biol Chem

Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.

Authors - Songhua Li
humanWB

Med Sci Monit

Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma.

Authors - Peng Liu
mouseWB,IF

PLoS One

Podoplanin increases the migration of human fibroblasts and affects the endothelial cell network formation: A possible role for cancer-associated fibroblasts in breast cancer progression.

Authors - Jaroslaw Suchanski
  • KD Validated
humanWB

Cancer Sci

Regulation of CD44 expression and focal adhesion by Golgi phosphatidylinositol 4-phosphate in breast cancer.

Authors - Takeshi Ijuin