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Mast Cell Chymase Polyclonal antibody

Mast Cell Chymase Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18189-1-AP

Synonyms

Alpha chymase, chymase, chymase 1, mast cell, CMA1, CYH, CYM, Mast Cell Chymase, Mast cell protease I, Mcpt 5, Mcpt5, MCT1



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, human placenta tissue, MCF-7 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18189-1-AP targets Mast Cell Chymase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Mast Cell Chymase fusion protein Ag12909
Full Name chymase 1, mast cell
Calculated Molecular Weight 247 aa, 27 kDa
Observed Molecular Weight 27-30 kDa
GenBank Accession NumberBC069370
Gene Symbol CMA1
Gene ID (NCBI) 1215
RRIDAB_2083611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.

Protocols

Product Specific Protocols
WB protocol for Mast Cell Chymase antibody 18189-1-APDownload protocol
IHC protocol for Mast Cell Chymase antibody 18189-1-APDownload protocol
IP protocol for Mast Cell Chymase antibody 18189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.

Authors - Emily Mackey
humanWB

Arterioscler Thromb Vasc Biol

Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide).

Authors - Wen Chen
human

Int J Mol Sci

Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.

Authors - Mika Yamanaka-Takaichi
humanWB

Cell Cycle

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.

Authors - Haoqing Shi
mouseWB

Sci Rep

Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.

Authors - Nicole Meyer
mouseWB

Immunology

The circadian clock is functional in eosinophils and mast cells.

Authors - Baumann Anja A