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UMP/CMP kinase Polyclonal antibody

UMP/CMP kinase Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11360-1-AP

Synonyms

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    WB analysis of mouse pancreas using 11360-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human pancreas tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    WB analysis of human pancreas using 11360-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    WB analysis of human skeletal muscle using 11360-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    WB analysis of human skeletal muscle using 11360-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    IHC staining of human lung cancer using 11360-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    IHC staining of human lung cancer using 11360-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    IHC staining of human breast cancer using 11360-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    IHC staining of human breast cancer using 11360-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UMP/CMP kinase Polyclonal antibody (11360-1-AP)

    IF Staining of HeLa using 11360-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11360-1-AP (UMP/CMP kinase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse pancreas tissue, human pancreas tissue, human skeletal muscle tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11360-1-AP targets UMP/CMP kinase in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UMP/CMP kinase fusion protein Ag1920
Full Name cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight 22-26 kDa
GenBank Accession NumberBC014961
Gene Symbol CMPK1
Gene ID (NCBI) 51727
RRIDAB_2081395
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

Protocols

Product Specific Protocols
WB protocol for UMP/CMP kinase antibody 11360-1-APDownload protocol
IHC protocol for UMP/CMP kinase antibody 11360-1-APDownload protocol
IF protocol for UMP/CMP kinase antibody 11360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Proteomics

Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry.

Authors - Víctor Llombart
View Article