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CNOT8 Polyclonal antibody

CNOT8 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10752-1-AP

Synonyms

CAF1, CAF1 like protein, CAF2, CALIF, CALIFp, CCR4 associated factor 8, CNOT8, hCAF1, POP2



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10752-1-AP targets CNOT8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNOT8 fusion protein Ag1201
Full Name CCR4-NOT transcription complex, subunit 8
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC017366
Gene symbol CNOT8
Gene ID (NCBI) 9337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.

Protocols

Product Specific Protocols
WB protocol for CNOT8 antibody 10752-1-APDownload protocol
IHC protocol for CNOT8 antibody 10752-1-APDownload protocol
IF protocol for CNOT8 antibody 10752-1-APDownload protocol
IP protocol for CNOT8 antibody 10752-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cells

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).

Authors - Tian-Li Duan
humanWB

Mol Biosyst

Large-scale investigation of oxygen response mutants in Saccharomyces cerevisiae.

Authors - Samanfar Bahram B
  • KD Validated