|Positive WB detected in||Jurkat cells, HeLa cells, mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human prostate cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10752-1-AP targets CNOT8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CNOT8 fusion protein Ag1201|
|Full Name||CCR4-NOT transcription complex, subunit 8|
|Calculated molecular weight||34 kDa|
|Observed molecular weight||34 kDa|
|GenBank accession number||BC017366|
|Gene ID (NCBI)||9337|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CCR4-NOT transcription complex subunit 8(CNOT8), is a ubiquitous transcription factor, which required for a diverse set of processes. The CCR4-NOT complex is a global regulator of RNA polymerase II, and functions as general transcription regulation complex. It consisted part by CCR4, NOT1 to NOT5, and CAF1. This is a rabbit polyclonal antibody raised against the full-length chain of human CNOT8.
|Product Specific Protocols|
|WB protocol for CNOT8 antibody 10752-1-AP||Download protocol|
|IHC protocol for CNOT8 antibody 10752-1-AP||Download protocol|
|IF protocol for CNOT8 antibody 10752-1-AP||Download protocol|
|IP protocol for CNOT8 antibody 10752-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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