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CPSF6 Polyclonal antibody

Name: CPSF6 Polyclonal antibody
Brand: Proteintech
SKU: 15489-1-AP
Rating: 5.00 (1 reviews)

CPSF6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 15489-1-AP

Synonyms

CFIM, CFIM68, CPSF 68 kDa subunit, CPSF6, HPBRII 4, HPBRII 7, Protein HPBRII 4/7

Validation Data Gallery

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AllWBIPIHCIF

WB analysis of HeLa using 15489-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis using 15489-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15489-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15489-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 15489-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15489-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15489-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15489-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 15489-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 15489-1-AP (CPSF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15489-1-AP
IP result of anti-CPSF6 (IP:15489-1-AP, 4ug; Detection:15489-1-AP 1:300) with HeLa cells lysate 3200ug.
IHC staining of human kidney using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15489-1-AP (CPSF6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 15489-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15489-1-AP (CPSF6 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of SW480 using 15489-1-AP
Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CPSF6 antibody (15489-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 15489-1-AP

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Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, Jurkat cells, PC-3 cells, MDA-MB-231 cells, SW480 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue, human heart tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	SW480 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below

Product Information

15489-1-AP targets CPSF6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CPSF6 fusion protein Ag7852
Full Name	cleavage and polyadenylation specific factor 6, 68kDa
Calculated Molecular Weight	59 kDa
Observed Molecular Weight	55-68 kDa
GenBank Accession Number	BC000714
Gene Symbol	CPSF6
Gene ID (NCBI)	11052
RRID	AB_10694140
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

Protocols

Product Specific Protocols
WB protocol for CPSF6 antibody 15489-1-AP	Download protocol
IHC protocol for CPSF6 antibody 15489-1-AP	Download protocol
IF protocol for CPSF6 antibody 15489-1-AP	Download protocol
IP protocol for CPSF6 antibody 15489-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell A Quantitative Genetic Interaction Map of HIV Infection. Authors - David E Gordon View Article
human	WB	PLoS Pathog In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects. Authors - Matthew S Henning View Article
human	WB	Elife Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Authors - Melissa Kane View Article
human	WB	J Virol Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz. Authors - Augustin Penda Twizerimana View Article
human	WB	iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. Authors - Mio Iwasaki View Article
human	WB	J Virol Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74. Authors - Akatsuki Saito KD Validated View Article

Reviews

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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Tatyana (Verified Customer) (03-11-2023)

The signal is not very bright but specific (siRNA knockdown verified). Predominantly nuclear, as expected. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; overnight incubation (no blocking step).

Applications: Immunofluorescence
Primary Antibody Dilution: 1:1000
Cell Tissue Type: Human, SH-SY5Y cells

CPSF6 Polyclonal antibody

CPSF6 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis of HeLa using 15489-1-AP

WB analysis using 15489-1-AP

WB analysis of Jurkat using 15489-1-AP

WB analysis of Jurkat using 15489-1-AP

WB analysis of Jurkat using 15489-1-AP

WB analysis of HeLa using 15489-1-AP

WB analysis of HeLa using 15489-1-AP

WB analysis of PC-3 using 15489-1-AP

WB analysis of PC-3 using 15489-1-AP

IP experiment of HeLa using 15489-1-AP

IHC staining of human kidney using 15489-1-AP

IHC staining of human kidney using 15489-1-AP

IHC staining of human liver using 15489-1-AP

IHC staining of human liver using 15489-1-AP

IHC staining of human heart using 15489-1-AP

IHC staining of human heart using 15489-1-AP

IF Staining of SW480 using 15489-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A Quantitative Genetic Interaction Map of HIV Infection.

In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Cell type-dependent escape of capsid inhibitors by simian immunodeficiency virus SIVcpz.

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.

Reviews