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  • KD/KO Validated

CPSF6 Antibody

CPSF6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15489-1-AP

Synonyms

CFIM, CFIM68, CPSF 68 kDa subunit, CPSF6, HPBRII 4, HPBRII 7, Protein HPBRII 4/7



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15489-1-AP targets CPSF6 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CPSF6 fusion protein Ag7852
Full Name cleavage and polyadenylation specific factor 6, 68kDa
Calculated molecular weight 59 kDa
Observed molecular weight 55-68 kDa
GenBank accession number BC000714
Gene symbol CPSF6
Gene ID (NCBI) 11052
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The binding of Cleavage factor Im (CFIM), also known as CPSF6, to the pre-mRNA is one of the earliest steps in the assembly of the cleavage and polyadenylation machinery and facilitates the recruitment of other processing factors. CFIM is required for the first step in pre-mRNA 3′-end processing and can be reconstituted in vitro from its heterologously expressed 25- and 68-kDa subunits. It involved in RNA binding, protein-protein interactions, and subcellular localization [PMID:15169763]. In addition, it is a pre-mRNA processing protein that dynamically shuttles between the nucleus and the cytoplasm and contains a C-terminal nuclear-targeting arginine/serine-rich (RS-) domain of the type bound by TNPO3[PMID:15169763,19864460].

Protocols

Product Specific Protocols
WB protocol for CPSF6 antibody 15489-1-APDownload protocol
IHC protocol for CPSF6 antibody 15489-1-APDownload protocol
IP protocol for CPSF6 antibody 15489-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Manag Res

NUDT21 Suppresses Breast Cancer Tumorigenesis Through Regulating CPSF6 Expression.

Authors - Bi-Jun Wang
  • KD Validated
humanWB

J Biol Chem

The HIV-1 capsid-binding host factor CPSF6 is post-transcriptionally regulated by the cellular microRNA miR-125b.

Authors - Evan Chaudhuri
  • KD Validated
humanWB

J Virol

Roles of capsid-interacting host factors in multimodal inhibition of HIV-1 by PF74.

Authors - Akatsuki Saito
  • KD Validated
humanWB

Elife

Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2.

Authors - Melissa Kane
humanWB

PLoS Pathog

In Vivo Functions of CPSF6 for HIV-1 as Revealed by HIV-1 Capsid Evolution in HLA-B27-Positive Subjects.

Authors - Matthew S Henning