CRABP1 Polyclonal antibody

CRABP1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12588-1-AP

Synonyms

CRABP, CRABP I, CRABP1, CRABPI, RBP5



Tested Applications

Positive WB detected inmouse embryo tissue, human spleen tissue, Transfected HEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12588-1-AP targets CRABP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRABP1 fusion protein Ag3291
Full Name cellular retinoic acid binding protein 1
Calculated Molecular Weight 137 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC022069
Gene Symbol CRABP1
Gene ID (NCBI) 1381
RRIDAB_2292271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cellular retinoic acid-binding proteins including CRABP1 and CRABP2 bind retinoic acid (RA), an important regulator of cell growth and differentiation, thus play an important role in RA-mediated differentiation and proliferation processes. It has been reported that CRABP1 influences the biological effects of RA in cell-selective manners by enhancing the physiological function of RA in keratinocytes or inhibiting RA-induced differentiation of neuroblastoma cells.

Protocols

Product Specific Protocols
WB protocol for CRABP1 antibody 12588-1-APDownload protocol
IHC protocol for CRABP1 antibody 12588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenIF

Elife

Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu
humanWB,IHC

Mol Oncol

CRABP-II methylation: a critical determinant of retinoic acid resistance of medulloblastoma cells.

Authors - Fu Yuan-Shan YS
humanWB

Cell Rep

Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.

Authors - Marie Kubota
mouseWB

Virol J

All-trans retinoic acid increases the pathogenicity of the H9N2 influenza virus in mice

Authors - Xiaofei Niu

Cell Rep

Dynamic interplay between IL-1 and WNT pathways in regulating dermal adipocyte lineage cells during skin development and wound regeneration

Authors - Lixiang Sun